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This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.

  • Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
  • This volume concentrates on Disease Models and Chemical Screens

1. Cover - p. 1 2. Title Page - p. 2 3. Table of Contents - p. 3 4. Copyright - p. 5 5. Dedication - p. 6 6. Contributors - p. 7 7. Chapter 1: Zebrafish Models of Germ Cell Tumor - p. 8 8. Chapter 2: Dissecting Mechanisms of Myelinated Axon Formation Using Zebrafish - p. 20 9. Chapter 3: In vivo Analysis of White Adipose Tissue in Zebrafish - p. 32 10. Chapter 4: Study of Host–Microbe Interactions in Zebrafish - p. 41 11. Chapter 5: Hematopoietic Stem Cell Development: Using the Zebrafish to Identify the Signaling Networks and Physical Forces Regulating Hematopoiesis - p. 55 12. Chapter 6: Zebrafish as a Model for Hemorrhagic Stroke - p. 65 13. Chapter 7: A Zebrafish Model for VHL and Hypoxia Signaling - p. 78 14. Chapter 8: Basement Membrane Diseases in Zebrafish - p. 91 15. Chapter 9: Zebrafish Provides a Novel Model for Lymphatic Vascular Research - p. 106 16. Chapter 10: Not All Bones are Created Equal – Using Zebrafish and Other Teleost Species in Osteogenesis Research - p. 114 17. Chapter 11: Zebrafish Assays of Ciliopathies - p. 123 18. Chapter 12: Infectious Disease Modeling and Innate Immune Function in Zebrafish Embryos - p. 131 19. Chapter 13: Zebrafish as a Model for the Study of Human Cancer - p. 148 20. Chapter 14: Generating and Analyzing Fish Models of Melanoma - p. 161 21. Chapter 15: Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System - p. 173 22. Chapter 16: Zebrafish Models of Rhabdomyosarcoma - p. 180 23. Chapter 17: Transplantation in Zebrafish - p. 188 24. Chapter 18: Disease Modeling by Gene Targeting Using MicroRNAs - p. 197 25. Chapter 19: Fluorescent Imaging of Cancer in Zebrafish - p. 205 26. Chapter 20: The Role of Fanconi Anemia/BRCA Genes in Zebrafish Sex Determination - p. 216 27. Chapter 21: Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery - p. 233 28. Chapter 22: Using the Zebrafish Photomotor Response for Psychotropic Drug Screening - p. 243 29. Chapter 23: Designing Zebrafish Chemical Screens - p. 245 30. Preface - p. 253 31. Index - p. 254 32. Volumes in Series - p. 255 33. Instructions for online access - p. 256

Descripcion
Descripción

This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.

  • Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
  • This volume concentrates on Disease Models and Chemical Screens

1. Cover - p. 1 2. Title Page - p. 2 3. Table of Contents - p. 3 4. Copyright - p. 5 5. Dedication - p. 6 6. Contributors - p. 7 7. Chapter 1: Zebrafish Models of Germ Cell Tumor - p. 8 8. Chapter 2: Dissecting Mechanisms of Myelinated Axon Formation Using Zebrafish - p. 20 9. Chapter 3: In vivo Analysis of White Adipose Tissue in Zebrafish - p. 32 10. Chapter 4: Study of Host–Microbe Interactions in Zebrafish - p. 41 11. Chapter 5: Hematopoietic Stem Cell Development: Using the Zebrafish to Identify the Signaling Networks and Physical Forces Regulating Hematopoiesis - p. 55 12. Chapter 6: Zebrafish as a Model for Hemorrhagic Stroke - p. 65 13. Chapter 7: A Zebrafish Model for VHL and Hypoxia Signaling - p. 78 14. Chapter 8: Basement Membrane Diseases in Zebrafish - p. 91 15. Chapter 9: Zebrafish Provides a Novel Model for Lymphatic Vascular Research - p. 106 16. Chapter 10: Not All Bones are Created Equal – Using Zebrafish and Other Teleost Species in Osteogenesis Research - p. 114 17. Chapter 11: Zebrafish Assays of Ciliopathies - p. 123 18. Chapter 12: Infectious Disease Modeling and Innate Immune Function in Zebrafish Embryos - p. 131 19. Chapter 13: Zebrafish as a Model for the Study of Human Cancer - p. 148 20. Chapter 14: Generating and Analyzing Fish Models of Melanoma - p. 161 21. Chapter 15: Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System - p. 173 22. Chapter 16: Zebrafish Models of Rhabdomyosarcoma - p. 180 23. Chapter 17: Transplantation in Zebrafish - p. 188 24. Chapter 18: Disease Modeling by Gene Targeting Using MicroRNAs - p. 197 25. Chapter 19: Fluorescent Imaging of Cancer in Zebrafish - p. 205 26. Chapter 20: The Role of Fanconi Anemia/BRCA Genes in Zebrafish Sex Determination - p. 216 27. Chapter 21: Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery - p. 233 28. Chapter 22: Using the Zebrafish Photomotor Response for Psychotropic Drug Screening - p. 243 29. Chapter 23: Designing Zebrafish Chemical Screens - p. 245 30. Preface - p. 253 31. Index - p. 254 32. Volumes in Series - p. 255 33. Instructions for online access - p. 256

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