Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter 1: FIB-SEM of mouse nervous tissue: Fast and slow sample preparation - p. xiii 8. Chapter 2: Expedited large-volume 3-D SEM workflows for comparative microanatomical imaging - p. 21 9. Chapter 3: Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM) - p. 39 10. Chapter 4: Serial block face-scanning electron microscopy for volume electron microscopy - p. 67 11. Chapter 5: Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events - p. 85 12. Chapter 6: Yeast membraneless compartments revealed by correlative light microscopy and electron tomography - p. 101 13. Chapter 7: In situ analysis of male meiosis in C. elegans - p. 117 14. Chapter 8: Software for automated acquisition of electron tomography tilt series - p. 134 15. Chapter 9: In situ cryo-electron tomography and subtomogram averaging of intraflagellar transport trains - p. 178 16. Chapter 10: CryoSTEM tomography in biology - p. 195 17. Chapter 11: Subtomogram averaging from cryo-electron tomograms - p. 215 18. Chapter 12: Computational methods for stitching, alignment, and artifact correction of serial section data - p. 259 19. Chapter 13: Content-aware image restoration for electron microscopy - p. 276
Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter 1: FIB-SEM of mouse nervous tissue: Fast and slow sample preparation - p. xiii 8. Chapter 2: Expedited large-volume 3-D SEM workflows for comparative microanatomical imaging - p. 21 9. Chapter 3: Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM) - p. 39 10. Chapter 4: Serial block face-scanning electron microscopy for volume electron microscopy - p. 67 11. Chapter 5: Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events - p. 85 12. Chapter 6: Yeast membraneless compartments revealed by correlative light microscopy and electron tomography - p. 101 13. Chapter 7: In situ analysis of male meiosis in C. elegans - p. 117 14. Chapter 8: Software for automated acquisition of electron tomography tilt series - p. 134 15. Chapter 9: In situ cryo-electron tomography and subtomogram averaging of intraflagellar transport trains - p. 178 16. Chapter 10: CryoSTEM tomography in biology - p. 195 17. Chapter 11: Subtomogram averaging from cryo-electron tomograms - p. 215 18. Chapter 12: Computational methods for stitching, alignment, and artifact correction of serial section data - p. 259 19. Chapter 13: Content-aware image restoration for electron microscopy - p. 276
