Transketolase, Volume 724 in the Methods in Enzymology series, is devoted to the description of cutting-edge methods to its study in biochemistry. Chapters in this new release include Role of transketolase-like 1 in human cortical neurogenesis, Transketolase in metabolic diseases, autoimmune diseases and cancer, Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatments, Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor, Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnostics, and much more.
Additional sections cover Transketolases in apicomplexan parasites, Electrochemical screening for microbial transketolase inhibitors identification, Transketolases isolation from plants: Diversity of pathways, Transketolase-sulfoglycolytic pathway, Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp., Biophysical and structural analysis of transketolases, Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro study, Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisons, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xix 8. Preface - p. xxv 9. Chapter One: Role of transketolase-like1 in human cortical neurogenesis - p. 5 10. Chapter Two: Transketolase in metabolic diseases, autoimmune diseases and cancer - p. 31 11. Chapter Three: Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor - p. 51 12. Chapter Four: Validation of the robust performance of novel transketolase epitopes for tuberculosis immunodiagnostics - p. 75 13. Chapter Five: Function and therapeutic potential of transketolase enzymes in parasitic protists - p. 97 14. Chapter Six: Electrochemical screening as a tool for identification of transketolase inhibitors - p. 117 15. Chapter Seven: Methods for studying plant transketolases - p. 153 16. Chapter Eight: Identification and functional characterization of transketolases in sulfoglycolytic pathways - p. 175 17. Chapter Nine: A split-gene transketolase essential for phosphonothrixin biosynthesis - p. 195 18. Chapter Ten: Biophysical and structural studies on transketolases - p. 221 19. Chapter Eleven: Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: In silico/in vitro study - p. 247 20. Chapter Twelve: Transketolase catalytic reaction pathway. Study of thiamine diphosphate activation and complete catalytic cycle - p. 285 21. Chapter Thirteen: Transketolase: Mechanistic aspects from QM and QM/MM investigations - p. 317 22. Chapter Fourteen: Transketolase enzyme discovery and engineering - p. 343 23. Chapter Fifteen: Evolved thermostable transketolase from Geobacillus stearothermophilus for the synthesis of deoxyketoses - p. 369 24. Chapter Sixteen: Practical high-throughput screening assays to engineer the substrate scope of transketolase variants - p. 399 25. Chapter Seventeen: Production of rare sugars by transketolase in combination with ketose 3-epimerase - p. 429 26. Chapter Eighteen: Engineering transketolase for stereoselective α-hydroxyketone synthesis - p. 447 27. Chapter Nineteen: Process considerations for the implementation and scale-up of transketolase-catalyzed biocatalytic processes - p. 473
Transketolase, Volume 724 in the Methods in Enzymology series, is devoted to the description of cutting-edge methods to its study in biochemistry. Chapters in this new release include Role of transketolase-like 1 in human cortical neurogenesis, Transketolase in metabolic diseases, autoimmune diseases and cancer, Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatments, Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor, Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnostics, and much more.
Additional sections cover Transketolases in apicomplexan parasites, Electrochemical screening for microbial transketolase inhibitors identification, Transketolases isolation from plants: Diversity of pathways, Transketolase-sulfoglycolytic pathway, Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp., Biophysical and structural analysis of transketolases, Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro study, Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisons, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xix 8. Preface - p. xxv 9. Chapter One: Role of transketolase-like1 in human cortical neurogenesis - p. 5 10. Chapter Two: Transketolase in metabolic diseases, autoimmune diseases and cancer - p. 31 11. Chapter Three: Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor - p. 51 12. Chapter Four: Validation of the robust performance of novel transketolase epitopes for tuberculosis immunodiagnostics - p. 75 13. Chapter Five: Function and therapeutic potential of transketolase enzymes in parasitic protists - p. 97 14. Chapter Six: Electrochemical screening as a tool for identification of transketolase inhibitors - p. 117 15. Chapter Seven: Methods for studying plant transketolases - p. 153 16. Chapter Eight: Identification and functional characterization of transketolases in sulfoglycolytic pathways - p. 175 17. Chapter Nine: A split-gene transketolase essential for phosphonothrixin biosynthesis - p. 195 18. Chapter Ten: Biophysical and structural studies on transketolases - p. 221 19. Chapter Eleven: Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: In silico/in vitro study - p. 247 20. Chapter Twelve: Transketolase catalytic reaction pathway. Study of thiamine diphosphate activation and complete catalytic cycle - p. 285 21. Chapter Thirteen: Transketolase: Mechanistic aspects from QM and QM/MM investigations - p. 317 22. Chapter Fourteen: Transketolase enzyme discovery and engineering - p. 343 23. Chapter Fifteen: Evolved thermostable transketolase from Geobacillus stearothermophilus for the synthesis of deoxyketoses - p. 369 24. Chapter Sixteen: Practical high-throughput screening assays to engineer the substrate scope of transketolase variants - p. 399 25. Chapter Seventeen: Production of rare sugars by transketolase in combination with ketose 3-epimerase - p. 429 26. Chapter Eighteen: Engineering transketolase for stereoselective α-hydroxyketone synthesis - p. 447 27. Chapter Nineteen: Process considerations for the implementation and scale-up of transketolase-catalyzed biocatalytic processes - p. 473
