Ubiquitination and Protein Stability - Part B, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Topics of note include chapters on Assays of SUMO protease function in mammalian cells, In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation, Methods to study proteasome regulatory particle assembly, Native mass spectrometry approaches to study the proteasome, Single-molecule methods to study the ubiquitin-proteasome system, Assays for the function of ubiquitin in the mammalian endocytic pathway, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Assays for protein retrotranslocation in ERAD - p. xvii 8. Chapter Two: Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae - p. 26 9. Chapter Three: Methodologies to monitor protein turnover at the inner nuclear membrane - p. 45 10. Chapter Four: Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 - p. 69 11. Chapter Five: Methods for genetic analysis of mammalian ER-associated degradation - p. 95 12. Chapter Six: A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies - p. 120 13. Chapter Seven: Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome - p. 143 14. Chapter Eight: Assays for ubiquitin-like protein ligation and proteasome function in archaea - p. 159 15. Chapter Nine: Structural mass spectrometry approaches to study the 20S proteasome - p. 178 16. Chapter Ten: Single-molecule methods for measuring ubiquitination and protein stability - p. 223 17. Chapter Eleven: In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation - p. 247 18. Chapter Twelve: Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway - p. 268 19. Chapter Thirteen: Detection of ubiquitinated targets in mammalian and Drosophila models - p. 291 20. Chapter Fourteen: Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans - p. 318 21. Chapter Fifteen: Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells - p. 336
Ubiquitination and Protein Stability - Part B, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Topics of note include chapters on Assays of SUMO protease function in mammalian cells, In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation, Methods to study proteasome regulatory particle assembly, Native mass spectrometry approaches to study the proteasome, Single-molecule methods to study the ubiquitin-proteasome system, Assays for the function of ubiquitin in the mammalian endocytic pathway, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Assays for protein retrotranslocation in ERAD - p. xvii 8. Chapter Two: Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae - p. 26 9. Chapter Three: Methodologies to monitor protein turnover at the inner nuclear membrane - p. 45 10. Chapter Four: Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 - p. 69 11. Chapter Five: Methods for genetic analysis of mammalian ER-associated degradation - p. 95 12. Chapter Six: A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies - p. 120 13. Chapter Seven: Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome - p. 143 14. Chapter Eight: Assays for ubiquitin-like protein ligation and proteasome function in archaea - p. 159 15. Chapter Nine: Structural mass spectrometry approaches to study the 20S proteasome - p. 178 16. Chapter Ten: Single-molecule methods for measuring ubiquitination and protein stability - p. 223 17. Chapter Eleven: In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation - p. 247 18. Chapter Twelve: Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway - p. 268 19. Chapter Thirteen: Detection of ubiquitinated targets in mammalian and Drosophila models - p. 291 20. Chapter Fourteen: Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans - p. 318 21. Chapter Fifteen: Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells - p. 336
