Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Author Biographies - p. ix 6. Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction - p. 1 7. Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers - p. 11 8. Chapter 3. Analyzing Your Template DNA and/or PCR Product - p. 31 9. Chapter 4. Quantitative PCR: Things to Consider - p. 45 10. Chapter 5. Carrying Out Q-PCR - p. 53 11. Chapter 6. Using PCR for Cloning and Protein Expression - p. 61 12. Chapter 7. Polymerase Chain Reaction for Knocking Out Genes - p. 73 13. Appendix 1 - p. 81 14. Appendix 2 - p. 83 15. Index - p. 85
Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Author Biographies - p. ix 6. Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction - p. 1 7. Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers - p. 11 8. Chapter 3. Analyzing Your Template DNA and/or PCR Product - p. 31 9. Chapter 4. Quantitative PCR: Things to Consider - p. 45 10. Chapter 5. Carrying Out Q-PCR - p. 53 11. Chapter 6. Using PCR for Cloning and Protein Expression - p. 61 12. Chapter 7. Polymerase Chain Reaction for Knocking Out Genes - p. 73 13. Appendix 1 - p. 81 14. Appendix 2 - p. 83 15. Index - p. 85
