Volume Electron Microscopy (vEM), Volume 177 is a collective term for a set of three-dimensional high-resolution ultrastructural imaging techniques that have delivered new insights into biological systems in recent years, garnering substantial interest in the life and clinical sciences. In this book, users will find a variety of vEM workflows and technologies, highlighting application areas with biologically relevant examples. Topics covered include Automated large volume sample preparation for vEM, Resin comparison for Serial Block Face Scanning Volume Electron Microscopy, Immunolabelling for SBF-SEM, Electron Microscopy in Plants, Serial section electron tomography, Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology, Array tomography, and much more.Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more. Includes chapters written by key leaders and developers in the field Provides detailed protocols, allowing for the application of workflows in one’s own laboratory setting Presents real tips and tricks you won't get from standard research papers
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. i 4. Series Page - p. ii 5. Copyright - p. iv 6. Contributors - p. xi 7. Preface - p. xvii 8. Chapter 1: Automated large volume sample preparation for vEM - p. 1 9. Chapter 2: Resin comparison for serial block face scanning volume electron microscopy - p. 33 10. Chapter 3: Nanogold based protein localization enables subcellular visualization of cell junction protein by SBF-SEM - p. 55 11. Chapter 4: A conventional fixation volume electron microscopy protocol for plants - p. 83 12. Chapter 5: Liver inter-organelle membrane contact sites revealed by serial section electron tomography - p. 101 13. Chapter 6: Neurons on tape: Automated Tape Collecting Ultramicrotomy-mediated volume EM for targeting neuropathology - p. 125 14. Chapter 7: A practical guide to starting SEM array tomography—An accessible volume EM technique - p. 171 15. Chapter 8: Mitochondria morphometry in 3D datasets obtained from mouse brains with serial block-face scanning electron microscopy - p. 197 16. Chapter 9: Serial block-face scanning electron microscopy of Schmidtea mediterranea - p. 213 17. Chapter 10: Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue - p. 241 18. Chapter 11: Methods of enhanced FIB-SEM sample preparation and image acquisition - p. 269 19. Chapter 12: Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy - p. 301 20. Chapter 13: A protocol for cryogenic volumetric imaging using serial plasma FIB/SEM - p. 327 21. Chapter 14: Toward scalable reuse of vEM data: OME-Zarr to the rescue - p. 359 22. Chapter 15: Public archiving of volume EM data - p. 389
Volume Electron Microscopy (vEM), Volume 177 is a collective term for a set of three-dimensional high-resolution ultrastructural imaging techniques that have delivered new insights into biological systems in recent years, garnering substantial interest in the life and clinical sciences. In this book, users will find a variety of vEM workflows and technologies, highlighting application areas with biologically relevant examples. Topics covered include Automated large volume sample preparation for vEM, Resin comparison for Serial Block Face Scanning Volume Electron Microscopy, Immunolabelling for SBF-SEM, Electron Microscopy in Plants, Serial section electron tomography, Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology, Array tomography, and much more.Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more. Includes chapters written by key leaders and developers in the field Provides detailed protocols, allowing for the application of workflows in one’s own laboratory setting Presents real tips and tricks you won't get from standard research papers
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. i 4. Series Page - p. ii 5. Copyright - p. iv 6. Contributors - p. xi 7. Preface - p. xvii 8. Chapter 1: Automated large volume sample preparation for vEM - p. 1 9. Chapter 2: Resin comparison for serial block face scanning volume electron microscopy - p. 33 10. Chapter 3: Nanogold based protein localization enables subcellular visualization of cell junction protein by SBF-SEM - p. 55 11. Chapter 4: A conventional fixation volume electron microscopy protocol for plants - p. 83 12. Chapter 5: Liver inter-organelle membrane contact sites revealed by serial section electron tomography - p. 101 13. Chapter 6: Neurons on tape: Automated Tape Collecting Ultramicrotomy-mediated volume EM for targeting neuropathology - p. 125 14. Chapter 7: A practical guide to starting SEM array tomography—An accessible volume EM technique - p. 171 15. Chapter 8: Mitochondria morphometry in 3D datasets obtained from mouse brains with serial block-face scanning electron microscopy - p. 197 16. Chapter 9: Serial block-face scanning electron microscopy of Schmidtea mediterranea - p. 213 17. Chapter 10: Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue - p. 241 18. Chapter 11: Methods of enhanced FIB-SEM sample preparation and image acquisition - p. 269 19. Chapter 12: Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy - p. 301 20. Chapter 13: A protocol for cryogenic volumetric imaging using serial plasma FIB/SEM - p. 327 21. Chapter 14: Toward scalable reuse of vEM data: OME-Zarr to the rescue - p. 359 22. Chapter 15: Public archiving of volume EM data - p. 389
