Biochemical Pathways and Environmental Responses in Plants, Part B, Volume 682 in the Methods in Enzymology series, highlights advances in the field with this new volume presenting chapters on MIE 681/682: Biochemical pathways and environmental responses in plants, Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, Biochemical characterization of plant aromatic aminotransferases, and much more.
Other chapters focus on Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Illustrations - p. iii 5. Series Page - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Preface - p. iii 9. Section I: Protein - p. iii 10. Systems and strategies for plant protein expression - p. iii 11. Biochemical characterization of plant aromatic aminotransferases - p. iii 12. Assaying plant formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based method - p. iii 13. Functional analysis of phosphoethanolamine N-methyltransferase in plants and parasites: Essential S-adenosylmethionine-dependent methyltransferase in choline and phospholipid metabolism - p. iii 14. An approach to nearest neighbor analysis of pigment-protein complexes using chemical cross-linking in combination with mass spectrometry - p. iii 15. Structural characterization of protein–DNA complexes using small angle X-ray scattering (SAXS) with contrast variation - p. iii 16. A fast and cost-effective procedure for reliable measurement of trypsin inhibitor activity in soy and soy products - p. iii 17. Section II: Metabolite - p. iii 18. Strategies to study the metabolic origins of specialized plant metabolites: The specialized 1,4-naphthoquinones - p. iii 19. A liquid chromatography-mass spectrometry-based metabolomics strategy to explore plant metabolic diversity - p. iii 20. Quantification of plant cardenolides by HPLC, measurement of Na+/K+-ATPase inhibition activity, and characterization of target enzymes - p. iii 21. Ex vivo metabolomics—A hypothesis-free approach to identify native substrate(s) and product(s) of orphan enzymes - p. iii 22. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation - p. iii 23. Section III: Environment - p. iii 24. Controlled environments for cannabis cultivation to support “omics” research studies and production - p. iii 25. Standard operating procedures for the comprehensive and reliable analysis of cannabis terpenes - p. iii 26. Isolation of novel chemical components and their plant target proteins under selenium stress - p. iii 27. Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing - p. iii 28. Analysis of plant flooding response - p. iii
Biochemical Pathways and Environmental Responses in Plants, Part B, Volume 682 in the Methods in Enzymology series, highlights advances in the field with this new volume presenting chapters on MIE 681/682: Biochemical pathways and environmental responses in plants, Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, Biochemical characterization of plant aromatic aminotransferases, and much more.
Other chapters focus on Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Illustrations - p. iii 5. Series Page - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Preface - p. iii 9. Section I: Protein - p. iii 10. Systems and strategies for plant protein expression - p. iii 11. Biochemical characterization of plant aromatic aminotransferases - p. iii 12. Assaying plant formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based method - p. iii 13. Functional analysis of phosphoethanolamine N-methyltransferase in plants and parasites: Essential S-adenosylmethionine-dependent methyltransferase in choline and phospholipid metabolism - p. iii 14. An approach to nearest neighbor analysis of pigment-protein complexes using chemical cross-linking in combination with mass spectrometry - p. iii 15. Structural characterization of protein–DNA complexes using small angle X-ray scattering (SAXS) with contrast variation - p. iii 16. A fast and cost-effective procedure for reliable measurement of trypsin inhibitor activity in soy and soy products - p. iii 17. Section II: Metabolite - p. iii 18. Strategies to study the metabolic origins of specialized plant metabolites: The specialized 1,4-naphthoquinones - p. iii 19. A liquid chromatography-mass spectrometry-based metabolomics strategy to explore plant metabolic diversity - p. iii 20. Quantification of plant cardenolides by HPLC, measurement of Na+/K+-ATPase inhibition activity, and characterization of target enzymes - p. iii 21. Ex vivo metabolomics—A hypothesis-free approach to identify native substrate(s) and product(s) of orphan enzymes - p. iii 22. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation - p. iii 23. Section III: Environment - p. iii 24. Controlled environments for cannabis cultivation to support “omics” research studies and production - p. iii 25. Standard operating procedures for the comprehensive and reliable analysis of cannabis terpenes - p. iii 26. Isolation of novel chemical components and their plant target proteins under selenium stress - p. iii 27. Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing - p. iii 28. Analysis of plant flooding response - p. iii
