Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.
Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Chapter 1: Pepsin-trypsin digested gliadin treatment in intestinal cells - p. 1 7. Chapter 2: Ex vivo gliadin stimulation of intestinal cells - p. 13 8. Chapter 3: Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers - p. 36 9. Chapter 4: Intestinal permeability assessment using lactulose and mannitol in celiac disease - p. 39 10. Chapter 5: In vivo sensitization to gliadin by oral administration - p. 51 11. Chapter 6: Separation of epithelial and immune cells from biopsy samples - p. 59 12. Chapter 7: Study and isolation of human intestinal dendritic cell and macrophage subsets - p. 69 13. Chapter 8: Elucidating the role of microbes in celiac disease through gnotobiotic modeling - p. 77 14. Chapter 9: In vitro differentiation of macrophages from peripheral blood cells of celiac patients - p. 103 15. Chapter 10: Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge - p. 113 16. Chapter 11: Isolation and cryopreservation of peripheral blood mononuclear cells - p. 127 17. Chapter 12: Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease - p. 143 18. Chapter 13: The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin - p. 159 19. Chapter 14: Human intestinal organoid models for celiac disease research - p. 173 20. Chapter 15: Preparation of pepsin trypsin digested gliadin for stimulation experiments - p. 195 21. Chapter 16: A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs - p. 203
Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.
Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Chapter 1: Pepsin-trypsin digested gliadin treatment in intestinal cells - p. 1 7. Chapter 2: Ex vivo gliadin stimulation of intestinal cells - p. 13 8. Chapter 3: Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers - p. 36 9. Chapter 4: Intestinal permeability assessment using lactulose and mannitol in celiac disease - p. 39 10. Chapter 5: In vivo sensitization to gliadin by oral administration - p. 51 11. Chapter 6: Separation of epithelial and immune cells from biopsy samples - p. 59 12. Chapter 7: Study and isolation of human intestinal dendritic cell and macrophage subsets - p. 69 13. Chapter 8: Elucidating the role of microbes in celiac disease through gnotobiotic modeling - p. 77 14. Chapter 9: In vitro differentiation of macrophages from peripheral blood cells of celiac patients - p. 103 15. Chapter 10: Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge - p. 113 16. Chapter 11: Isolation and cryopreservation of peripheral blood mononuclear cells - p. 127 17. Chapter 12: Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease - p. 143 18. Chapter 13: The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin - p. 159 19. Chapter 14: Human intestinal organoid models for celiac disease research - p. 173 20. Chapter 15: Preparation of pepsin trypsin digested gliadin for stimulation experiments - p. 195 21. Chapter 16: A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs - p. 203
