Clinical Microbiology, Volume 664 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS‐CoV‐2, Activity-based probes for bacterial histidine kinases, Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more.
Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xiii 8. Chapter One: Integration of bioinformatic and chemoproteomic tools for the study of enzyme conservation in closely related bacterial species - p. 1 9. Chapter Two: A ligand selection strategy to customize small molecule probes for activity-based protein profiling (LS-ABPP) - p. 23 10. Chapter Three: Activity-based ATP analog probes for bacterial histidine kinases - p. 59 11. Chapter Four: BSH-TRAP: Bile salt hydrolase tagging and retrieval with activity-based probes - p. 85 12. Chapter Five: Detecting and identifying glycoside hydrolases using cyclophellitol-derived activity-based probes - p. 103 13. Chapter Six: Chemical proteomics for identifying short-chain fatty acid modified proteins in Salmonella - p. 135 14. Chapter Seven: Kinetic dissection of macromolecular complex formation with minimally perturbing fluorescent probes - p. 151 15. Chapter Eight: Mobilization of cryptic antibiotic biosynthesis loci from human-pathogenic Nocardia - p. 173 16. Chapter Nine: Assessing and utilizing esterase specificity in antimicrobial prodrug development - p. 199 17. Chapter Ten: Identification of volatile producing enzymes in higher fungi: Combining analytical and bioinformatic methods - p. 221 18. Chapter Eleven: Bile salt hydrolase profiling by fluorogenic probes in the human gut microbiome - p. 243 19. Chapter Twelve: Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria - p. 267
Clinical Microbiology, Volume 664 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS‐CoV‐2, Activity-based probes for bacterial histidine kinases, Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more.
Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xiii 8. Chapter One: Integration of bioinformatic and chemoproteomic tools for the study of enzyme conservation in closely related bacterial species - p. 1 9. Chapter Two: A ligand selection strategy to customize small molecule probes for activity-based protein profiling (LS-ABPP) - p. 23 10. Chapter Three: Activity-based ATP analog probes for bacterial histidine kinases - p. 59 11. Chapter Four: BSH-TRAP: Bile salt hydrolase tagging and retrieval with activity-based probes - p. 85 12. Chapter Five: Detecting and identifying glycoside hydrolases using cyclophellitol-derived activity-based probes - p. 103 13. Chapter Six: Chemical proteomics for identifying short-chain fatty acid modified proteins in Salmonella - p. 135 14. Chapter Seven: Kinetic dissection of macromolecular complex formation with minimally perturbing fluorescent probes - p. 151 15. Chapter Eight: Mobilization of cryptic antibiotic biosynthesis loci from human-pathogenic Nocardia - p. 173 16. Chapter Nine: Assessing and utilizing esterase specificity in antimicrobial prodrug development - p. 199 17. Chapter Ten: Identification of volatile producing enzymes in higher fungi: Combining analytical and bioinformatic methods - p. 221 18. Chapter Eleven: Bile salt hydrolase profiling by fluorogenic probes in the human gut microbiome - p. 243 19. Chapter Twelve: Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria - p. 267
