Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Tables - p. iii 5. Series Page - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Preface - p. iii 9. How to apply the broad toolbox of correlative light and electron microscopy to address a specific biological question - p. iii 10. Some tips and tricks for a Correlative Light Electron Microscopy workflow using stable expression of fluorescent proteins - p. iii 11. Targeting of membrane proteins with fluoronanogold probes for high-resolution correlative microscopy - p. iii 12. Correlative light and electron microscopy at defined cell cycle stages in a controlled environment - p. iii 13. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopy - p. iii 14. A correlated light and electron microscopy approach to study the subcellular localization of phosphorylated vimentin in human lung tissue - p. iii 15. Array tomography of in vivo labeled synaptic receptors - p. iii 16. Correlative cryo-microscopy pipelines for in situ cellular studies - p. iii 17. Building a super-resolution fluorescence cryomicroscope - p. iii 18. Imaging intracellular components in situ using super-resolution cryo-correlative light and electron microscopy - p. iii 19. Toward quantitative super-resolution methods for cryo-CLEM - p. iii 20. Laboratory based correlative cryo-soft X-ray tomography and cryo-fluorescence microscopy - p. iii
Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. List of Tables - p. iii 5. Series Page - p. iii 6. Copyright - p. iii 7. Contributors - p. iii 8. Preface - p. iii 9. How to apply the broad toolbox of correlative light and electron microscopy to address a specific biological question - p. iii 10. Some tips and tricks for a Correlative Light Electron Microscopy workflow using stable expression of fluorescent proteins - p. iii 11. Targeting of membrane proteins with fluoronanogold probes for high-resolution correlative microscopy - p. iii 12. Correlative light and electron microscopy at defined cell cycle stages in a controlled environment - p. iii 13. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopy - p. iii 14. A correlated light and electron microscopy approach to study the subcellular localization of phosphorylated vimentin in human lung tissue - p. iii 15. Array tomography of in vivo labeled synaptic receptors - p. iii 16. Correlative cryo-microscopy pipelines for in situ cellular studies - p. iii 17. Building a super-resolution fluorescence cryomicroscope - p. iii 18. Imaging intracellular components in situ using super-resolution cryo-correlative light and electron microscopy - p. iii 19. Toward quantitative super-resolution methods for cryo-CLEM - p. iii 20. Laboratory based correlative cryo-soft X-ray tomography and cryo-fluorescence microscopy - p. iii
