Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins.
Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. iv 7. Preface - p. xix 8. Section 1 Post-transcriptional modification enzymes - p. xxii 9. Chapter One: D-Seq: Genome-wide detection of dihydrouridine modifications in RNA - p. 3 10. Chapter Two: Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA - p. 23 11. Chapter Three: Base-resolution quantitative DAMM-seq for mapping RNA methylations in tRNA and mitochondrial polycistronic RNA - p. 39 12. Chapter Four: Discovering RNA modification enzymes using a comparative genomics approach - p. 55 13. Chapter Five: Functional analysis of tRNA modification enzymes using mutational profiling - p. 69 14. Chapter Six: Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins - p. 103 15. Chapter Seven: Enzymatic synthesis of RNA standards for mapping and quantifying RNA modifications in sequencing analysis - p. 127 16. Section 2 Nucleases - p. 153 17. Chapter Eight: Single-cell analysis of RNase L‐mediated mRNA decay - p. 157 18. Chapter Nine: Characterization of RNase J - p. 177 19. Chapter Ten: Pri-miRNA cleavage assays for the Microprocessor complex - p. 217 20. Chapter Eleven: The pre-miRNA cleavage assays for DICER - p. 231 21. Chapter Twelve: Methods for the study of ribonuclease targeting chimeras (RiboTACs) - p. 249 22. Chapter Thirteen: Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus - p. 299
Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins.
Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. iv 7. Preface - p. xix 8. Section 1 Post-transcriptional modification enzymes - p. xxii 9. Chapter One: D-Seq: Genome-wide detection of dihydrouridine modifications in RNA - p. 3 10. Chapter Two: Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA - p. 23 11. Chapter Three: Base-resolution quantitative DAMM-seq for mapping RNA methylations in tRNA and mitochondrial polycistronic RNA - p. 39 12. Chapter Four: Discovering RNA modification enzymes using a comparative genomics approach - p. 55 13. Chapter Five: Functional analysis of tRNA modification enzymes using mutational profiling - p. 69 14. Chapter Six: Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins - p. 103 15. Chapter Seven: Enzymatic synthesis of RNA standards for mapping and quantifying RNA modifications in sequencing analysis - p. 127 16. Section 2 Nucleases - p. 153 17. Chapter Eight: Single-cell analysis of RNase L‐mediated mRNA decay - p. 157 18. Chapter Nine: Characterization of RNase J - p. 177 19. Chapter Ten: Pri-miRNA cleavage assays for the Microprocessor complex - p. 217 20. Chapter Eleven: The pre-miRNA cleavage assays for DICER - p. 231 21. Chapter Twelve: Methods for the study of ribonuclease targeting chimeras (RiboTACs) - p. 249 22. Chapter Thirteen: Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus - p. 299
