Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated.
Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry.
1. Essentials in Modern HPLC Separations - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Preface - p. Page v 7. List of Illustrations - p. Page vi 8. List of Tables - p. Page vii 9. Chapter 1 : Introductory information regarding HPLC - p. Page vii 10. Chapter 2 : Overview of HPLC instrumentation and its use - p. Page 19 11. Chapter 3 : Parameters for the characterization of HPLC separation - p. Page 59 12. Chapter 4 : Equilibrium types in HPLC - p. Page 103 13. Chapter 5 : Intermolecular interactions - p. Page 143 14. Chapter 6 : Characterization of analytes and matrices - p. Page 176 15. Chapter 7 : Mobile phases and their properties - p. Page 203 16. Chapter 8 : Analytical HPLC columns and their characteristics - p. Page 264 17. Chapter 9 : Reversed-phase HPLC - p. Page 330 18. Chapter 10 : Other HPLC separations performed on hydrophobic stationary phases - p. Page 410 19. Chapter 11 : Hydrophilic interaction liquid chromatography - p. Page 444 20. Chapter 12 : Other HPLC separations performed on polar stationary phases - p. Page 474 21. Chapter 13 : Ion exchange, ion-moderated, and ligand exchange liquid chromatography - p. Page 483 22. Chapter 14 : Chiral HPLC separations - p. Page 510 23. Chapter 15 : Size exclusion HPLC - p. Page 534 24. Chapter 16 : Affinity, immunoaffinity, and aptamer type HPLC - p. Page 556 25. Chapter 17 : Mixed-mode HPLC - p. Page 567 26. Chapter 18 : Utilization of HPLC in chemical analysis - p. Page 575 27. Appendix to Chapter 6 - p. Page 593 28. Appendix to chapter 7 - p. Page 593 29. Appendix to chapter 8 - p. Page 593 30. Appendix to chapter 9 - p. Page 593 31. Appendix to chapter 11 - p. Page 593 32. Appendix to chapter 13 - p. Page 593 33. Appendix to chapter 14 - p. Page 593 34. Appendix to chapter 15 - p. Page 593 35. Index - p. Page 593
Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated.
Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry.
1. Essentials in Modern HPLC Separations - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Preface - p. Page v 7. List of Illustrations - p. Page vi 8. List of Tables - p. Page vii 9. Chapter 1 : Introductory information regarding HPLC - p. Page vii 10. Chapter 2 : Overview of HPLC instrumentation and its use - p. Page 19 11. Chapter 3 : Parameters for the characterization of HPLC separation - p. Page 59 12. Chapter 4 : Equilibrium types in HPLC - p. Page 103 13. Chapter 5 : Intermolecular interactions - p. Page 143 14. Chapter 6 : Characterization of analytes and matrices - p. Page 176 15. Chapter 7 : Mobile phases and their properties - p. Page 203 16. Chapter 8 : Analytical HPLC columns and their characteristics - p. Page 264 17. Chapter 9 : Reversed-phase HPLC - p. Page 330 18. Chapter 10 : Other HPLC separations performed on hydrophobic stationary phases - p. Page 410 19. Chapter 11 : Hydrophilic interaction liquid chromatography - p. Page 444 20. Chapter 12 : Other HPLC separations performed on polar stationary phases - p. Page 474 21. Chapter 13 : Ion exchange, ion-moderated, and ligand exchange liquid chromatography - p. Page 483 22. Chapter 14 : Chiral HPLC separations - p. Page 510 23. Chapter 15 : Size exclusion HPLC - p. Page 534 24. Chapter 16 : Affinity, immunoaffinity, and aptamer type HPLC - p. Page 556 25. Chapter 17 : Mixed-mode HPLC - p. Page 567 26. Chapter 18 : Utilization of HPLC in chemical analysis - p. Page 575 27. Appendix to Chapter 6 - p. Page 593 28. Appendix to chapter 7 - p. Page 593 29. Appendix to chapter 8 - p. Page 593 30. Appendix to chapter 9 - p. Page 593 31. Appendix to chapter 11 - p. Page 593 32. Appendix to chapter 13 - p. Page 593 33. Appendix to chapter 14 - p. Page 593 34. Appendix to chapter 15 - p. Page 593 35. Index - p. Page 593
