Laboratory Methods in Dynamic Electroanalysis is a useful guide to introduce analytical chemists and scientists of related disciplines to the world of dynamic electroanalysis using simple and low-cost methods. The trend toward decentralization of analysis has made this fascinating field one of the fastest-growing branches of analytical chemistry. As electroanalytical devices have moved from conventional electrochemical cells (10-20 mL) to current cells (e.g. 5-50 mL) based on different materials such as paper or polymers that integrate thick- or thin-film electrodes, interesting strategies have emerged, such as the combination of microfluidic cells and biosensing or nanostructuration of electrodes.
This book provides detailed, easy procedures for dynamic electroanalysis and covers the main trends in electrochemical cells and electrodes, including microfluidic electrodes, electrochemical detection in microchip electrophoresis, nanostructuration of electrodes, development of bio (enzymatic, immuno, and DNA) assays, paper-based electrodes, interdigitated array electrodes, multiplexed analysis, and combination with optics. Different strategies and techniques (amperometric, voltammetric, and impedimetric) are presented in a didactic, practice-based way, and a bibliography provides readers with additional sources of information.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Dedication - p. v 6. Contributors - p. xv 7. Preface - p. xvii 8. Acknowledgments - p. xxi 9. Chapter 1. Dynamic electroanalysis: an overview - p. 1 10. I. Dynamic electroanalytical techniques - p. 10 11. Chapter 2. Determination of ascorbic acid in dietary supplements by cyclic voltammetry - p. 13 12. Chapter 3. Electrochemical behavior of the redox probe hexaammineruthenium(III) ([Ru(NH3)6]3+) using voltammetric techniques - p. 25 13. Chapter 4. Anodic stripping voltammetric determination of lead and cadmium with stencil-printed transparency electrodes - p. 35 14. Chapter 5. Adsorptive stripping voltammetry of indigo blue in a flow system - p. 47 15. Chapter 6. Enhancing electrochemical performance by using redox cycling with interdigitated electrodes - p. 57 16. Chapter 7. Amperometric detection of NADH using carbon-based electrodes - p. 67 17. Chapter 8. Chronoamperometric determination of ascorbic acid on paper-based devices - p. 75 18. Chapter 9. Electrochemical detection of melatonin in a flow injection analysis system - p. 85 19. Chapter 10. Batch injection analysis for amperometric determination of ascorbic acid at ruthenium dioxide screen-printed electrodes - p. 99 20. Chapter 11. Impedimetric aptasensor for determination of the antibiotic neomycin B - p. 109 21. Chapter 12. Electrochemical impedance spectroscopy for characterization of electrode surfaces: carbon nanotubes on gold electrodes - p. 119 22. II. Electroanalysis and microfluidics - p. 126 23. Chapter 13. Single- and dual-channel hybrid PDMS/glass microchip electrophoresis device with amperometric detection - p. 129 24. Chapter 14. Analysis of uric acid and related compounds in urine samples by electrophoresis in microfluidic chips - p. 139 25. Chapter 15. Microchannel modifications in microchip reverse electrophoresis for ferrocene carboxylic acid determination - p. 151 26. Chapter 16. Integrated microfluidic electrochemical sensors to enhance automated flow analysis systems - p. 161 27. III. Bioelectroanalysis - p. 170 28. Chapter 17. Bienzymatic amperometric glucose biosensor - p. 173 29. Chapter 18. Determination of ethyl alcohol in beverages using an electrochemical enzymatic sensor - p. 183 30. Chapter 19. Enzymatic determination of ethanol on screen-printed cobalt phthalocyanine/carbon electrodes - p. 193 31. Chapter 20. Immunoelectroanalytical assay based on the electrocatalytic effect of gold labels on silver electrodeposition - p. 201 32. Chapter 21. Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence - p. 213 33. Chapter 22. Aptamer-based magnetoassay for gluten determination - p. 221 34. IV. Nanomaterials and electroanalysis - p. 229 35. Chapter 23. Determination of lead with electrodes nanostructured with gold nanoparticles - p. 233 36. Chapter 24. Electrochemical behavior of the dye methylene blue on screen-printed gold electrodes modified with carbon nanotubes - p. 243 37. V. Low-cost electroanalysis - p. 254 38. Chapter 25. Determination of glucose with an enzymatic paper-based sensor - p. 257 39. Chapter 26. Determination of arsenic (III) in wines with nanostructured paper-based electrodes - p. 267 40. Chapter 27. Pin-based electrochemical sensor - p. 277 41. Chapter 28. Flow injection electroanalysis with pins - p. 287 42. Chapter 29. Staple-based paper electrochemical platform for quantitative analysis - p. 297 43. VI. Multiplexed electroanalysis - p. 303 44. Chapter 30. Simultaneous measurements with a multiplexed platform containing eight electrochemical cells - p. 307 45. Chapter 31. Simultaneous detection of bacteria causing community-acquired pneumonia by genosensing - p. 317 46. VII. Spectroelectrochemical techniques - p. 326 47. Chapter 32. Electrochemiluminescence of tris (1,10-phenanthroline) ruthenium(II) complex with multipulsed amperometric detection - p. 329 48. Chapter 33. Detection of hydrogen peroxide by flow injection analysis based on electrochemiluminescence resonance energy transfer donor–acceptor strategy - p. 339 49. Chapter 34. Determination of tris(bipyridine)ruthenium(II) based on electrochemical surface-enhanced raman scattering - p. 349 50. VIII. General considerations - p. 357 51. Chapter 35. Design of experiments at electroanalysis. Application to the optimization of nanostructured electrodes for sensor development - p. 361 52. Chapter 36. Bibliographic resources in electroanalysis - p. 373 53. Index - p. 379
Laboratory Methods in Dynamic Electroanalysis is a useful guide to introduce analytical chemists and scientists of related disciplines to the world of dynamic electroanalysis using simple and low-cost methods. The trend toward decentralization of analysis has made this fascinating field one of the fastest-growing branches of analytical chemistry. As electroanalytical devices have moved from conventional electrochemical cells (10-20 mL) to current cells (e.g. 5-50 mL) based on different materials such as paper or polymers that integrate thick- or thin-film electrodes, interesting strategies have emerged, such as the combination of microfluidic cells and biosensing or nanostructuration of electrodes.
This book provides detailed, easy procedures for dynamic electroanalysis and covers the main trends in electrochemical cells and electrodes, including microfluidic electrodes, electrochemical detection in microchip electrophoresis, nanostructuration of electrodes, development of bio (enzymatic, immuno, and DNA) assays, paper-based electrodes, interdigitated array electrodes, multiplexed analysis, and combination with optics. Different strategies and techniques (amperometric, voltammetric, and impedimetric) are presented in a didactic, practice-based way, and a bibliography provides readers with additional sources of information.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iv 5. Dedication - p. v 6. Contributors - p. xv 7. Preface - p. xvii 8. Acknowledgments - p. xxi 9. Chapter 1. Dynamic electroanalysis: an overview - p. 1 10. I. Dynamic electroanalytical techniques - p. 10 11. Chapter 2. Determination of ascorbic acid in dietary supplements by cyclic voltammetry - p. 13 12. Chapter 3. Electrochemical behavior of the redox probe hexaammineruthenium(III) ([Ru(NH3)6]3+) using voltammetric techniques - p. 25 13. Chapter 4. Anodic stripping voltammetric determination of lead and cadmium with stencil-printed transparency electrodes - p. 35 14. Chapter 5. Adsorptive stripping voltammetry of indigo blue in a flow system - p. 47 15. Chapter 6. Enhancing electrochemical performance by using redox cycling with interdigitated electrodes - p. 57 16. Chapter 7. Amperometric detection of NADH using carbon-based electrodes - p. 67 17. Chapter 8. Chronoamperometric determination of ascorbic acid on paper-based devices - p. 75 18. Chapter 9. Electrochemical detection of melatonin in a flow injection analysis system - p. 85 19. Chapter 10. Batch injection analysis for amperometric determination of ascorbic acid at ruthenium dioxide screen-printed electrodes - p. 99 20. Chapter 11. Impedimetric aptasensor for determination of the antibiotic neomycin B - p. 109 21. Chapter 12. Electrochemical impedance spectroscopy for characterization of electrode surfaces: carbon nanotubes on gold electrodes - p. 119 22. II. Electroanalysis and microfluidics - p. 126 23. Chapter 13. Single- and dual-channel hybrid PDMS/glass microchip electrophoresis device with amperometric detection - p. 129 24. Chapter 14. Analysis of uric acid and related compounds in urine samples by electrophoresis in microfluidic chips - p. 139 25. Chapter 15. Microchannel modifications in microchip reverse electrophoresis for ferrocene carboxylic acid determination - p. 151 26. Chapter 16. Integrated microfluidic electrochemical sensors to enhance automated flow analysis systems - p. 161 27. III. Bioelectroanalysis - p. 170 28. Chapter 17. Bienzymatic amperometric glucose biosensor - p. 173 29. Chapter 18. Determination of ethyl alcohol in beverages using an electrochemical enzymatic sensor - p. 183 30. Chapter 19. Enzymatic determination of ethanol on screen-printed cobalt phthalocyanine/carbon electrodes - p. 193 31. Chapter 20. Immunoelectroanalytical assay based on the electrocatalytic effect of gold labels on silver electrodeposition - p. 201 32. Chapter 21. Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence - p. 213 33. Chapter 22. Aptamer-based magnetoassay for gluten determination - p. 221 34. IV. Nanomaterials and electroanalysis - p. 229 35. Chapter 23. Determination of lead with electrodes nanostructured with gold nanoparticles - p. 233 36. Chapter 24. Electrochemical behavior of the dye methylene blue on screen-printed gold electrodes modified with carbon nanotubes - p. 243 37. V. Low-cost electroanalysis - p. 254 38. Chapter 25. Determination of glucose with an enzymatic paper-based sensor - p. 257 39. Chapter 26. Determination of arsenic (III) in wines with nanostructured paper-based electrodes - p. 267 40. Chapter 27. Pin-based electrochemical sensor - p. 277 41. Chapter 28. Flow injection electroanalysis with pins - p. 287 42. Chapter 29. Staple-based paper electrochemical platform for quantitative analysis - p. 297 43. VI. Multiplexed electroanalysis - p. 303 44. Chapter 30. Simultaneous measurements with a multiplexed platform containing eight electrochemical cells - p. 307 45. Chapter 31. Simultaneous detection of bacteria causing community-acquired pneumonia by genosensing - p. 317 46. VII. Spectroelectrochemical techniques - p. 326 47. Chapter 32. Electrochemiluminescence of tris (1,10-phenanthroline) ruthenium(II) complex with multipulsed amperometric detection - p. 329 48. Chapter 33. Detection of hydrogen peroxide by flow injection analysis based on electrochemiluminescence resonance energy transfer donor–acceptor strategy - p. 339 49. Chapter 34. Determination of tris(bipyridine)ruthenium(II) based on electrochemical surface-enhanced raman scattering - p. 349 50. VIII. General considerations - p. 357 51. Chapter 35. Design of experiments at electroanalysis. Application to the optimization of nanostructured electrodes for sensor development - p. 361 52. Chapter 36. Bibliographic resources in electroanalysis - p. 373 53. Index - p. 379
