Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Dedication - p. page_v 6. Preface to the First Edition - p. page_ix 7. Preface to the Second Edition - p. page_xi 8. Preface to the Third Edition - p. page_xiii 9. Chapter 1: The Relation Between the Object and the Image - p. page_xiv 10. Chapter 2: The Geometric Relationship Between Object and Image - p. page_15 11. Chapter 3: The Dependence of Image Formation on the Nature of Light - p. page_50 12. Chapter 4: Bright-Field Microscopy - p. page_94 13. Chapter 5: Photomicrography - p. page_116 14. Chapter 6: Methods of Generating Contrast - p. page_132 15. Chapter 7: Fluorescence Microscopy - p. page_150 16. Chapter 8: Superresolution - p. page_170 17. Chapter 9: Polarization Microscopy - p. page_188 18. Abstract - p. page_189 19. What Is Polarized Light? - p. page_189 20. Use an Analyzer to Test for Polarized Light - p. page_192 21. Production of Polarized Light - p. page_194 22. Influencing Light - p. page_200 23. Design of a Polarizing Microscope - p. page_201 24. What Is the Molecular Basis of Birefringence? - p. page_206 25. Interference of Polarized Light - p. page_210 26. The Origin of Colors in Birefringent Specimens - p. page_218 27. Use of Compensators to Determine the Magnitude and Sign of Birefringence - p. page_219 28. Pleochroism - p. page_231 29. Crystalline Versus Form Birefringence - p. page_231 30. Orthoscopic Versus Conoscopic Observations - p. page_232 31. Reflected Light Polarization Microscopy - p. page_232 32. Uses of Polarization Microscopy - p. page_232 33. Optical Rotatory (or Rotary) Polarization and Optical Rotatory (or Rotary) Dispersion - p. page_235 34. Web Resources - p. page_242 35. Chapter 10: Interference Microscopy - p. page_242 36. Chapter 11: Differential Interference Contrast (DIC) Microscopy - p. page_296 37. Chapter 12: Amplitude Modulation Contrast Microscopy - p. page_307 38. Chapter 13: Video and Digital Microscopy - p. page_316 39. Chapter 14: Image Processing and Analysis - p. page_334 40. Chapter 15: Various Types of Microscopes and Accessories - p. page_354 41. Chapter 16: Laboratory Exercises - p. page_382 42. Appendix A: Light Microscopy: A Retrospective - p. page_400 43. Appendix B: A Final Exam - p. page_410 44. Appendix C: A Microscopist’s Model of the Photon - p. page_413 45. Appendix D: The Kirchhoff Diffraction Equation Based on the Binary Photon - p. page_424 46. The Kirchhoff Diffraction Integral Based On the Binary Photon - p. page_426 47. The Two-Dimensional Diffraction Pattern for Apertures of Various Forms - p. page_434 48. The Three-Dimensional Diffraction Pattern for a Point Source - p. page_437 49. The Description of Plane Waves - p. page_440 50. Summary - p. page_442 51. References - p. page_443 52. Index - p. page_487
Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Dedication - p. page_v 6. Preface to the First Edition - p. page_ix 7. Preface to the Second Edition - p. page_xi 8. Preface to the Third Edition - p. page_xiii 9. Chapter 1: The Relation Between the Object and the Image - p. page_xiv 10. Chapter 2: The Geometric Relationship Between Object and Image - p. page_15 11. Chapter 3: The Dependence of Image Formation on the Nature of Light - p. page_50 12. Chapter 4: Bright-Field Microscopy - p. page_94 13. Chapter 5: Photomicrography - p. page_116 14. Chapter 6: Methods of Generating Contrast - p. page_132 15. Chapter 7: Fluorescence Microscopy - p. page_150 16. Chapter 8: Superresolution - p. page_170 17. Chapter 9: Polarization Microscopy - p. page_188 18. Abstract - p. page_189 19. What Is Polarized Light? - p. page_189 20. Use an Analyzer to Test for Polarized Light - p. page_192 21. Production of Polarized Light - p. page_194 22. Influencing Light - p. page_200 23. Design of a Polarizing Microscope - p. page_201 24. What Is the Molecular Basis of Birefringence? - p. page_206 25. Interference of Polarized Light - p. page_210 26. The Origin of Colors in Birefringent Specimens - p. page_218 27. Use of Compensators to Determine the Magnitude and Sign of Birefringence - p. page_219 28. Pleochroism - p. page_231 29. Crystalline Versus Form Birefringence - p. page_231 30. Orthoscopic Versus Conoscopic Observations - p. page_232 31. Reflected Light Polarization Microscopy - p. page_232 32. Uses of Polarization Microscopy - p. page_232 33. Optical Rotatory (or Rotary) Polarization and Optical Rotatory (or Rotary) Dispersion - p. page_235 34. Web Resources - p. page_242 35. Chapter 10: Interference Microscopy - p. page_242 36. Chapter 11: Differential Interference Contrast (DIC) Microscopy - p. page_296 37. Chapter 12: Amplitude Modulation Contrast Microscopy - p. page_307 38. Chapter 13: Video and Digital Microscopy - p. page_316 39. Chapter 14: Image Processing and Analysis - p. page_334 40. Chapter 15: Various Types of Microscopes and Accessories - p. page_354 41. Chapter 16: Laboratory Exercises - p. page_382 42. Appendix A: Light Microscopy: A Retrospective - p. page_400 43. Appendix B: A Final Exam - p. page_410 44. Appendix C: A Microscopist’s Model of the Photon - p. page_413 45. Appendix D: The Kirchhoff Diffraction Equation Based on the Binary Photon - p. page_424 46. The Kirchhoff Diffraction Integral Based On the Binary Photon - p. page_426 47. The Two-Dimensional Diffraction Pattern for Apertures of Various Forms - p. page_434 48. The Three-Dimensional Diffraction Pattern for a Point Source - p. page_437 49. The Description of Plane Waves - p. page_440 50. Summary - p. page_442 51. References - p. page_443 52. Index - p. page_487
