The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology. Methods in iPSC Technology, Volume 9 addresses the different methods used for induced pluripotent stem cell (iPSC) formation, maintenance, expansion, and differentiation. The ability to reprogram different cell types to iPSCs offers an opportunity to generate pluripotent patient-specific cell lines that can help in the understanding of multiple human disorders. This volume addresses a variety of current methods used in the generation and manipulation of iPSCs, such as magnetic nanoparticles, piggyBac vectors, lentiviral vectors, bioscaffolds, somatic cell nuclear transfer, CRISPR/Cas9, bacteria, and much more. This volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and organ transplantation and is contributed by world-renowned authors in the field.
1. Methods in iPSC Technology, Volume 9 - p. i 2. Cover - p. i 3. Advances in Stem Cell Biology - p. ii 4. Title Page - p. iii 5. Copyright Page - p. iv 6. Dedication - p. v 7. Table of Contents - p. xiii 8. About the editor - p. xvi 9. Preface - p. xvi 10. Chapter 1 Current reprogramming methods to generate high-quality iPSCs - p. 2 11. Chapter 2 Using magnetic nanoparticles in iPSCs - p. 38 12. Chapter 3 PiggyBac vectors in pluripotent stem cell research and applications - p. 56 13. Chapter 4 Lentiviral vectors as the delivery vehicles for transduction into iPSCs - p. 80 14. Chapter 5 Decellularized liver extracellular matrix for iPSC-based liver engineering - p. 102 15. Chapter 6 Combining bioscaffolds and iPSCs in the treatment of neural trauma and Alzheimer’s disease - p. 124 16. Chapter 7 Emerging strategies for scalable human induced pluripotent stem cell expansion and differentiation - p. 164 17. Chapter 8 One plus one could be greater than two - p. 188 18. Chapter 9 Bacteria to form induced pluripotent stem cells - p. 232 19. Chapter 10 CRISPR/Cas9 technologies to manipulate human induced pluripotent stem cells - p. 250 20. Chapter 11 Scalable expansion of human pluripotent stem cells for biomanufacturing cellular therapeutics - p. 290 21. Chapter 12 Xeno-free cultivation of human induced pluripotent stem cells for clinical applications - p. 310 22. Index - p. 343
The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology. Methods in iPSC Technology, Volume 9 addresses the different methods used for induced pluripotent stem cell (iPSC) formation, maintenance, expansion, and differentiation. The ability to reprogram different cell types to iPSCs offers an opportunity to generate pluripotent patient-specific cell lines that can help in the understanding of multiple human disorders. This volume addresses a variety of current methods used in the generation and manipulation of iPSCs, such as magnetic nanoparticles, piggyBac vectors, lentiviral vectors, bioscaffolds, somatic cell nuclear transfer, CRISPR/Cas9, bacteria, and much more. This volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and organ transplantation and is contributed by world-renowned authors in the field.
1. Methods in iPSC Technology, Volume 9 - p. i 2. Cover - p. i 3. Advances in Stem Cell Biology - p. ii 4. Title Page - p. iii 5. Copyright Page - p. iv 6. Dedication - p. v 7. Table of Contents - p. xiii 8. About the editor - p. xvi 9. Preface - p. xvi 10. Chapter 1 Current reprogramming methods to generate high-quality iPSCs - p. 2 11. Chapter 2 Using magnetic nanoparticles in iPSCs - p. 38 12. Chapter 3 PiggyBac vectors in pluripotent stem cell research and applications - p. 56 13. Chapter 4 Lentiviral vectors as the delivery vehicles for transduction into iPSCs - p. 80 14. Chapter 5 Decellularized liver extracellular matrix for iPSC-based liver engineering - p. 102 15. Chapter 6 Combining bioscaffolds and iPSCs in the treatment of neural trauma and Alzheimer’s disease - p. 124 16. Chapter 7 Emerging strategies for scalable human induced pluripotent stem cell expansion and differentiation - p. 164 17. Chapter 8 One plus one could be greater than two - p. 188 18. Chapter 9 Bacteria to form induced pluripotent stem cells - p. 232 19. Chapter 10 CRISPR/Cas9 technologies to manipulate human induced pluripotent stem cells - p. 250 20. Chapter 11 Scalable expansion of human pluripotent stem cells for biomanufacturing cellular therapeutics - p. 290 21. Chapter 12 Xeno-free cultivation of human induced pluripotent stem cells for clinical applications - p. 310 22. Index - p. 343
