Modifications and Targeting of Protein Termini, Part B, Volume 686 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including In vitro production of N-degron fused proteins and its application, Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomics, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathway. Other chapters cover Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, Building libraries to dissect terminal degrons with fluorescent timers, Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS, Probing the effects of N-terminal acetylation on α-synuclein structure, aggregation and toxicity, Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE), and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Updated release includes the latest information on Modifications and Targeting of Protein Termini
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface – The impact of protein N- and C-terminal modifications - p. xv 7. Chapter One: Increasing the coverage of the N-terminome with LysN amino terminal enrichment (LATE) - p. 1 8. Chapter Two: Using cell lysates to assess N-terminal acetyltransferase activity and impairment - p. 29 9. Chapter Three: Probing the effects of N-terminal acetylation on α-synuclein structure, aggregation and cytotoxicity - p. 45 10. Chapter Four: Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry - p. 85 11. Chapter Five: In vitro production of N-degron fused proteins and its application - p. 99 12. Chapter Six: TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini - p. 125 13. Chapter Seven: Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS - p. 143 14. Chapter Eight: Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway - p. 165 15. Chapter Nine: In vitro autoubiquitination activity of E3 ubiquitin ligases of the N-degron pathway - p. 205 16. Chapter Ten: Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae - p. 221 17. Chapter Eleven: Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin - p. 235 18. Chapter Twelve: Monitoring ADO dependent proteolysis in cells using fluorescent reporter proteins - p. 267 19. Chapter Thirteen: Building yeast libraries to dissect terminal degrons with fluorescent timers - p. 297 20. Chapter Fourteen: Multiplexed protein stability (MPS) profiling of terminal degrons using fluorescent timer libraries in Saccharomyces cerevisiae - p. 335 21. Chapter Fifteen: Characterization of degradation signals at protein C-termini - p. 345
Modifications and Targeting of Protein Termini, Part B, Volume 686 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including In vitro production of N-degron fused proteins and its application, Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomics, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathway. Other chapters cover Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, Building libraries to dissect terminal degrons with fluorescent timers, Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS, Probing the effects of N-terminal acetylation on α-synuclein structure, aggregation and toxicity, Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE), and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Updated release includes the latest information on Modifications and Targeting of Protein Termini
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface – The impact of protein N- and C-terminal modifications - p. xv 7. Chapter One: Increasing the coverage of the N-terminome with LysN amino terminal enrichment (LATE) - p. 1 8. Chapter Two: Using cell lysates to assess N-terminal acetyltransferase activity and impairment - p. 29 9. Chapter Three: Probing the effects of N-terminal acetylation on α-synuclein structure, aggregation and cytotoxicity - p. 45 10. Chapter Four: Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry - p. 85 11. Chapter Five: In vitro production of N-degron fused proteins and its application - p. 99 12. Chapter Six: TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini - p. 125 13. Chapter Seven: Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS - p. 143 14. Chapter Eight: Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway - p. 165 15. Chapter Nine: In vitro autoubiquitination activity of E3 ubiquitin ligases of the N-degron pathway - p. 205 16. Chapter Ten: Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae - p. 221 17. Chapter Eleven: Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin - p. 235 18. Chapter Twelve: Monitoring ADO dependent proteolysis in cells using fluorescent reporter proteins - p. 267 19. Chapter Thirteen: Building yeast libraries to dissect terminal degrons with fluorescent timers - p. 297 20. Chapter Fourteen: Multiplexed protein stability (MPS) profiling of terminal degrons using fluorescent timer libraries in Saccharomyces cerevisiae - p. 335 21. Chapter Fifteen: Characterization of degradation signals at protein C-termini - p. 345
