Nanoarmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes, Volume 590 is the latest volume in the Methods in Enzymology series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated information on a variety of topics, including specific chapters on Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One, Enzyme Adsorption on Nanoparticle Surfaces Probed by Highly Sensitive Second Harmonic Light Scattering, Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method, and Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts.
Users will find this to be an all-encompassing resource on nanoarmoring in enzymes.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xi 7. Chapter One: Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One - p. xix 8. Chapter Two: Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering - p. 31 9. Chapter Three: Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method - p. 58 10. Chapter Four: Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts - p. 75 11. Chapter Five: Strategies for Biophysical Characterization of Protein–Polymer Conjugates - p. 91 12. Chapter Six: Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis - p. 114 13. Chapter Seven: Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform - p. 141 14. Chapter Eight: Armored Enzyme–Nanohybrids and Their Catalytic Function Under Challenging Conditions - p. 167 15. Chapter Nine: Approaches for Conjugating Tailor-Made Polymers to Proteins - p. 192 16. Chapter Ten: NanoArmoring of Enzymes by Polymer-Functionalized Iron Oxide Nanoparticles - p. 224 17. Chapter Eleven: Expression of Cellulolytic Enzyme as a Fusion Protein That Reacts Specifically With a Polymeric Scaffold - p. 257 18. Chapter Twelve: Nanoarmoring of Proteins by Conjugation to Block Copolymer Micelles - p. 276 19. Chapter Thirteen: Semisynthetic Enzymes by Protein–Peptide Site-Directed Covalent Conjugation: Methods and Applications - p. 304 20. Chapter Fourteen: Transgultaminase-Mediated Nanoarmoring of Enzymes by PEGylation - p. 316 21. Chapter Fifteen: Polymer-Based Protein Engineering: Synthesis and Characterization of Armored, High Graft Density Polymer–Protein Conjugates - p. 346 22. Chapter Sixteen: Nano-Armoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes - p. 380 23. Chapter Seventeen: Nanoarmored Enzymes for Organic Enzymology: Synthesis and Characterization of Poly(2-Alkyloxazoline)–Enzyme Conjugates - p. 411 24. Chapter Eighteen: Preparation and Applications of Dendronized Polymer–Enzyme Conjugates - p. 444 25. Chapter Nineteen: Nanoarmoring of Enzymes by Interlocking in Cellulose Fibers With Poly(Acrylic Acid) - p. 474 26. Author Index - p. 501 27. Subject Index - p. 527
Nanoarmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes, Volume 590 is the latest volume in the Methods in Enzymology series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated information on a variety of topics, including specific chapters on Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One, Enzyme Adsorption on Nanoparticle Surfaces Probed by Highly Sensitive Second Harmonic Light Scattering, Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method, and Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts.
Users will find this to be an all-encompassing resource on nanoarmoring in enzymes.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xi 7. Chapter One: Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One - p. xix 8. Chapter Two: Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering - p. 31 9. Chapter Three: Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method - p. 58 10. Chapter Four: Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts - p. 75 11. Chapter Five: Strategies for Biophysical Characterization of Protein–Polymer Conjugates - p. 91 12. Chapter Six: Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis - p. 114 13. Chapter Seven: Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform - p. 141 14. Chapter Eight: Armored Enzyme–Nanohybrids and Their Catalytic Function Under Challenging Conditions - p. 167 15. Chapter Nine: Approaches for Conjugating Tailor-Made Polymers to Proteins - p. 192 16. Chapter Ten: NanoArmoring of Enzymes by Polymer-Functionalized Iron Oxide Nanoparticles - p. 224 17. Chapter Eleven: Expression of Cellulolytic Enzyme as a Fusion Protein That Reacts Specifically With a Polymeric Scaffold - p. 257 18. Chapter Twelve: Nanoarmoring of Proteins by Conjugation to Block Copolymer Micelles - p. 276 19. Chapter Thirteen: Semisynthetic Enzymes by Protein–Peptide Site-Directed Covalent Conjugation: Methods and Applications - p. 304 20. Chapter Fourteen: Transgultaminase-Mediated Nanoarmoring of Enzymes by PEGylation - p. 316 21. Chapter Fifteen: Polymer-Based Protein Engineering: Synthesis and Characterization of Armored, High Graft Density Polymer–Protein Conjugates - p. 346 22. Chapter Sixteen: Nano-Armoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes - p. 380 23. Chapter Seventeen: Nanoarmored Enzymes for Organic Enzymology: Synthesis and Characterization of Poly(2-Alkyloxazoline)–Enzyme Conjugates - p. 411 24. Chapter Eighteen: Preparation and Applications of Dendronized Polymer–Enzyme Conjugates - p. 444 25. Chapter Nineteen: Nanoarmoring of Enzymes by Interlocking in Cellulose Fibers With Poly(Acrylic Acid) - p. 474 26. Author Index - p. 501 27. Subject Index - p. 527
