Plasma Membrane Shaping summarizes current knowledge on how cells shape their membrane. Organized in four sections, the book opens with a broad overview of the plasma membrane, its composition, usual shapes and substructures, Actin/WASP/arp2/3 structures, BAR domains, and Ankyrin repeat domains, dynamin, and phospholipid signaling. Other sections cover the shaping of the plasma membrane for transport processes, discussions on exosomes, microvesicles, and endosomes, clathrin-coated pits, caveolae, and other endocytic pits, membrane deformation for cell movement, and some of the most current dry and wet lab research techniques to investigate cellular membrane shaping.
This is an ideal resource for new researchers coming into this area as well as for graduate students. The methods section will be of interest to both microscopists and computer scientists dedicated to the visualization, data collection, and analysis of plasma membrane shaping experiments.
1. Chapter 1. Introduction and overview of the book - p. 1 2. Section 1: Building blocks and the shape builders - p. 3 3. Chapter 2. Membrane lipid compositions and their difference between subcellular structures - p. 7 4. Chapter 3. Wiskott–Aldrich syndrome protein family, linking cellular signaling to the actin cytoskeleton - p. 27 5. Chapter 4. BAR domains - p. 47 6. Chapter 5. Ankyrin repeat domains with an amphipathic helix for membrane deformation - p. 65 7. Chapter 6. Dynamin: molecular scissors for membrane fission - p. 77 8. Chapter 7. ESCRT-mediated plasma membrane shaping - p. 91 9. Chapter 8. Phospholipid signaling, lipase - p. 105 10. Chapter 9. Phospholipid signaling: phosphoinositide kinases and phosphatases - p. 119 11. Chapter 10. Plasma membrane shaping by protein phase separation - p. 139 12. Chapter 11. Synthetic mimics of membrane-active proteins and peptides - p. 159 13. Section 2: The functions of the plasma membrane substance and derived vesicles - p. 170 14. Chapter 12. The extracellular vesicles - p. 177 15. Chapter 13. Regulation of membrane traffic through recycling endosomes by membrane phospholipid phosphatidylserine - p. 193 16. Chapter 14. Membrane shaping for clathrin-coated pits and endocytosis - p. 205 17. Chapter 15. Caveolae biogenesis and lipid sorting at the plasma membrane - p. 219 18. Chapter 16. Non-vesicular lipid transport at membrane contact sites between the endoplasmic reticulum and the plasma membrane - p. 229 19. Chapter 17. Lamellipodia and filopodia - p. 245 20. Chapter 18. Membrane structures, dynamics, and shaping in invadopodia and podosomes - p. 265 21. Section 3: Force around the plasma membrane and imaging - p. 274 22. Chapter 19. Membrane tension and mechanobiology of cell migration - p. 281 23. Chapter 20. Brownian ratchet force sensor at the contacting point between F-actin barbed end and lamellipodium tip plasma membrane - p. 295 24. Chapter 21. Biophysics of cellular membrane shaping on fiber networks - p. 307 25. Chapter 22. Reconstitution of membrane symmetry breaking - p. 333 26. Section 4: The microscopy and image analysis for understanding the shaping of the membrane - p. 350 27. Chapter 23. Imaging three-dimensional dynamics of plasma membrane structures using ultrathin plane illumination microscopy - p. 357 28. Chapter 24. Deep learning for cell shape analysis - p. 375 29. Section 5: The essentiality of the membrane shaping by theory and reconstitution - p. 388 30. Chapter 25. Physical principles of cellular membrane shapes - p. 393 31. Chapter 26. Modeling cellular shape changes in the presence of curved membrane proteins and active cytoskeletal forces - p. 415 32. Chapter 27. Molecular dynamics - p. 431 33. Index - p. 445
Plasma Membrane Shaping summarizes current knowledge on how cells shape their membrane. Organized in four sections, the book opens with a broad overview of the plasma membrane, its composition, usual shapes and substructures, Actin/WASP/arp2/3 structures, BAR domains, and Ankyrin repeat domains, dynamin, and phospholipid signaling. Other sections cover the shaping of the plasma membrane for transport processes, discussions on exosomes, microvesicles, and endosomes, clathrin-coated pits, caveolae, and other endocytic pits, membrane deformation for cell movement, and some of the most current dry and wet lab research techniques to investigate cellular membrane shaping.
This is an ideal resource for new researchers coming into this area as well as for graduate students. The methods section will be of interest to both microscopists and computer scientists dedicated to the visualization, data collection, and analysis of plasma membrane shaping experiments.
1. Chapter 1. Introduction and overview of the book - p. 1 2. Section 1: Building blocks and the shape builders - p. 3 3. Chapter 2. Membrane lipid compositions and their difference between subcellular structures - p. 7 4. Chapter 3. Wiskott–Aldrich syndrome protein family, linking cellular signaling to the actin cytoskeleton - p. 27 5. Chapter 4. BAR domains - p. 47 6. Chapter 5. Ankyrin repeat domains with an amphipathic helix for membrane deformation - p. 65 7. Chapter 6. Dynamin: molecular scissors for membrane fission - p. 77 8. Chapter 7. ESCRT-mediated plasma membrane shaping - p. 91 9. Chapter 8. Phospholipid signaling, lipase - p. 105 10. Chapter 9. Phospholipid signaling: phosphoinositide kinases and phosphatases - p. 119 11. Chapter 10. Plasma membrane shaping by protein phase separation - p. 139 12. Chapter 11. Synthetic mimics of membrane-active proteins and peptides - p. 159 13. Section 2: The functions of the plasma membrane substance and derived vesicles - p. 170 14. Chapter 12. The extracellular vesicles - p. 177 15. Chapter 13. Regulation of membrane traffic through recycling endosomes by membrane phospholipid phosphatidylserine - p. 193 16. Chapter 14. Membrane shaping for clathrin-coated pits and endocytosis - p. 205 17. Chapter 15. Caveolae biogenesis and lipid sorting at the plasma membrane - p. 219 18. Chapter 16. Non-vesicular lipid transport at membrane contact sites between the endoplasmic reticulum and the plasma membrane - p. 229 19. Chapter 17. Lamellipodia and filopodia - p. 245 20. Chapter 18. Membrane structures, dynamics, and shaping in invadopodia and podosomes - p. 265 21. Section 3: Force around the plasma membrane and imaging - p. 274 22. Chapter 19. Membrane tension and mechanobiology of cell migration - p. 281 23. Chapter 20. Brownian ratchet force sensor at the contacting point between F-actin barbed end and lamellipodium tip plasma membrane - p. 295 24. Chapter 21. Biophysics of cellular membrane shaping on fiber networks - p. 307 25. Chapter 22. Reconstitution of membrane symmetry breaking - p. 333 26. Section 4: The microscopy and image analysis for understanding the shaping of the membrane - p. 350 27. Chapter 23. Imaging three-dimensional dynamics of plasma membrane structures using ultrathin plane illumination microscopy - p. 357 28. Chapter 24. Deep learning for cell shape analysis - p. 375 29. Section 5: The essentiality of the membrane shaping by theory and reconstitution - p. 388 30. Chapter 25. Physical principles of cellular membrane shapes - p. 393 31. Chapter 26. Modeling cellular shape changes in the presence of curved membrane proteins and active cytoskeletal forces - p. 415 32. Chapter 27. Molecular dynamics - p. 431 33. Index - p. 445
