Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xix 8. Chapter One: X-ray crystallography shines a light on pore-forming toxins - p. 1 9. Chapter Two: How protein engineering has revealed the molecular mechanisms of pore-forming toxins - p. 47 10. Chapter Three: Electron microscopy as a critical tool in the determination of pore forming mechanisms in proteins - p. 71 11. Chapter Four: Going full circle: Determining the structures of complement component 9 - p. 103 12. Chapter Five: Preparation of Clostridium perfringens binary iota-toxin pore complex for structural analysis using cryo-EM - p. 125 13. Chapter Six: AFM imaging of pore forming proteins - p. 149 14. Chapter Seven: High-speed atomic force microscopy to study pore-forming proteins - p. 189 15. Chapter Eight: The use of giant unilamellar vesicles to study functional properties of pore-forming toxins - p. 219 16. Chapter Nine: Preparation and utility of asymmetric lipid vesicles for studies of perfringolysin O-lipid interactions - p. 253 17. Chapter Ten: Molecular basis for the activation of actinoporins by lipids - p. 277 18. Chapter Eleven: Biophysical approaches to study actinoporin-lipid interactions - p. 307 19. Chapter Twelve: Conformational switching, refolding and membrane insertion of the diphtheria toxin translocation domain - p. 341 20. Chapter Thirteen: Cysteine-based crosslinking approach for characterization of oligomeric pore-forming proteins in the mitochondrial membranes - p. 371 21. Chapter Fourteen: Topological analysis of type 3 secretion translocons in native membranes - p. 397 22. Chapter Fifteen: Single-molecule imaging of pore-forming toxin dynamics in droplet interface bilayers - p. 431 23. Chapter Sixteen: Using multiscale molecular dynamics simulations to obtain insights into pore forming toxin mechanisms - p. 461 24. Chapter Seventeen: The use of pore-forming toxins to image lipids and lipid domains - p. 506 25. Chapter Eighteen: The use of anthrolysin O and ostreolysin A to study cholesterol in cell membranes - p. 543 26. Chapter Nineteen: Strategies for enzymological studies and measurements of biological molecules with the cytolysin A nanopore - p. 567 27. Chapter Twenty: Pore-forming toxins as tools for polymer analytics: From sizing to sequencing - p. 587 28. Chapter Twenty-One: Use of pore-forming toxins to study co-translocational protein folding - p. 635
Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. xix 8. Chapter One: X-ray crystallography shines a light on pore-forming toxins - p. 1 9. Chapter Two: How protein engineering has revealed the molecular mechanisms of pore-forming toxins - p. 47 10. Chapter Three: Electron microscopy as a critical tool in the determination of pore forming mechanisms in proteins - p. 71 11. Chapter Four: Going full circle: Determining the structures of complement component 9 - p. 103 12. Chapter Five: Preparation of Clostridium perfringens binary iota-toxin pore complex for structural analysis using cryo-EM - p. 125 13. Chapter Six: AFM imaging of pore forming proteins - p. 149 14. Chapter Seven: High-speed atomic force microscopy to study pore-forming proteins - p. 189 15. Chapter Eight: The use of giant unilamellar vesicles to study functional properties of pore-forming toxins - p. 219 16. Chapter Nine: Preparation and utility of asymmetric lipid vesicles for studies of perfringolysin O-lipid interactions - p. 253 17. Chapter Ten: Molecular basis for the activation of actinoporins by lipids - p. 277 18. Chapter Eleven: Biophysical approaches to study actinoporin-lipid interactions - p. 307 19. Chapter Twelve: Conformational switching, refolding and membrane insertion of the diphtheria toxin translocation domain - p. 341 20. Chapter Thirteen: Cysteine-based crosslinking approach for characterization of oligomeric pore-forming proteins in the mitochondrial membranes - p. 371 21. Chapter Fourteen: Topological analysis of type 3 secretion translocons in native membranes - p. 397 22. Chapter Fifteen: Single-molecule imaging of pore-forming toxin dynamics in droplet interface bilayers - p. 431 23. Chapter Sixteen: Using multiscale molecular dynamics simulations to obtain insights into pore forming toxin mechanisms - p. 461 24. Chapter Seventeen: The use of pore-forming toxins to image lipids and lipid domains - p. 506 25. Chapter Eighteen: The use of anthrolysin O and ostreolysin A to study cholesterol in cell membranes - p. 543 26. Chapter Nineteen: Strategies for enzymological studies and measurements of biological molecules with the cytolysin A nanopore - p. 567 27. Chapter Twenty: Pore-forming toxins as tools for polymer analytics: From sizing to sequencing - p. 587 28. Chapter Twenty-One: Use of pore-forming toxins to study co-translocational protein folding - p. 635
