Redox Chemistry and Biology of Thiols offers an applied, comprehensive overview of redox chemistry and biology of thiol-dependent processes. Running from basic biology and chemistry of redox phenomena to research methods and highlighting recently identified roles of thiols across cellular and bodily systems, this book draws upon a range of disciplines to illuminate new research directions, new applications of thiol studies, and clinical translation. Sections cover thiol oxidizing species, thiol reactivity and modifications, thioredoxin, glutaredoxin, glutathione, peroxidases, thiol repair enzymes, thiol oxidative signaling, disulfide bond formation, thiol-based redox biosensors, cysteine and hydrogen sulfide metabolism, iron-sulfur cluster biogenesis, thiols in chloroplasts, blood thiols, sugar and polyamine thiols in pathogenic organisms, redox medicine (therapeutic applications of thiols and drug development), as well as methods and bioinformatics tools.
1. Redox Chemistry and Biology of Thiols - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page i 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Cover Credit - p. Page v 7. Contributors - p. Page v 8. Preface - p. Page viii 9. Acknowledgments - p. Page x 10. List of Illustrations - p. Page xi 11. List of Tables - p. Page xii 12. Chapter 1 : Basic concepts of thiol chemistry and biology - p. Page xii 13. Chapter 2 : Chemical basis of cysteine reactivity and specificity: Acidity and nucleophilicity - p. Page 17 14. Chapter 3 : Computational functional analysis of cysteine residues in proteins - p. Page 51 15. Chapter 4 : Global approaches for protein thiol redox state detection and quantification - p. Page 77 16. Chapter 5 : Thiol oxidation by biologically-relevant reactive species - p. Page 97 17. Chapter 6 : Thiyl radicals: Formation, properties, and detection - p. Page 107 18. Chapter 7 : Detection of the oxidation products of thiols: Disulfides, and sulfenic, sulfinic, and sulfonic acids - p. Page 127 19. Chapter 8 : Biochemistry and detection of S-nitrosothiols - p. Page 149 20. Chapter 9 : Thiol modification and signaling by biological electrophiles - p. Page 169 21. Chapter 10 : Thioredoxin and glutathione reductases - p. Page 190 22. Chapter 11 : Functional plasticity in the thioredoxin family: FeS-thio- and glutaredoxins☆ - p. Page 209 23. Chapter 12 : Glutathione and glutathione-dependent enzymes - p. Page 232 24. Chapter 13 : Thiol- and selenol-based peroxidases: Structure and catalytic properties - p. Page 261 25. Chapter 14 : Thiol peroxidase-based redox relays - p. Page 294 26. Chapter 15 : Compartmentalized disulfide bond formation pathways - p. Page 316 27. Chapter 16 : Disulfide bond formation in Escherichia coli - p. Page 336 28. Chapter 17 : Thiol-based redox probes - p. Page 361 29. Chapter 18 : Selenocysteine-containing proteins - p. Page 396 30. Chapter 19 : Overview of cysteine metabolism - p. Page 418 31. Chapter 20 : Hydrogen sulfide and persulfides - p. Page 441 32. Chapter 21 : The role of thiols in iron–sulfur cluster biogenesis - p. Page 477 33. Chapter 22 : Thiol-based redox control in chloroplasts - p. Page 499 34. Chapter 23 : Sugar-based cysteine thiols recruited for oxidative stress defense and redox regulation - p. Page 525 35. Chapter 24 : Polyamine-based thiols in pathogens - p. Page 549 36. Chapter 25 : Thiols in blood - p. Page 572 37. Chapter 26 : A thiol chemistry perspective on redox medicine - p. Page 604 38. Chapter 27 : Therapeutic applications of low-molecular-weight thiols and selenocompounds - p. Page 635 39. Chapter 28 : Thiol targets in drug development to combat bacterial infections - p. Page 664 40. Index - p. Page 699
Redox Chemistry and Biology of Thiols offers an applied, comprehensive overview of redox chemistry and biology of thiol-dependent processes. Running from basic biology and chemistry of redox phenomena to research methods and highlighting recently identified roles of thiols across cellular and bodily systems, this book draws upon a range of disciplines to illuminate new research directions, new applications of thiol studies, and clinical translation. Sections cover thiol oxidizing species, thiol reactivity and modifications, thioredoxin, glutaredoxin, glutathione, peroxidases, thiol repair enzymes, thiol oxidative signaling, disulfide bond formation, thiol-based redox biosensors, cysteine and hydrogen sulfide metabolism, iron-sulfur cluster biogenesis, thiols in chloroplasts, blood thiols, sugar and polyamine thiols in pathogenic organisms, redox medicine (therapeutic applications of thiols and drug development), as well as methods and bioinformatics tools.
1. Redox Chemistry and Biology of Thiols - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page i 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Cover Credit - p. Page v 7. Contributors - p. Page v 8. Preface - p. Page viii 9. Acknowledgments - p. Page x 10. List of Illustrations - p. Page xi 11. List of Tables - p. Page xii 12. Chapter 1 : Basic concepts of thiol chemistry and biology - p. Page xii 13. Chapter 2 : Chemical basis of cysteine reactivity and specificity: Acidity and nucleophilicity - p. Page 17 14. Chapter 3 : Computational functional analysis of cysteine residues in proteins - p. Page 51 15. Chapter 4 : Global approaches for protein thiol redox state detection and quantification - p. Page 77 16. Chapter 5 : Thiol oxidation by biologically-relevant reactive species - p. Page 97 17. Chapter 6 : Thiyl radicals: Formation, properties, and detection - p. Page 107 18. Chapter 7 : Detection of the oxidation products of thiols: Disulfides, and sulfenic, sulfinic, and sulfonic acids - p. Page 127 19. Chapter 8 : Biochemistry and detection of S-nitrosothiols - p. Page 149 20. Chapter 9 : Thiol modification and signaling by biological electrophiles - p. Page 169 21. Chapter 10 : Thioredoxin and glutathione reductases - p. Page 190 22. Chapter 11 : Functional plasticity in the thioredoxin family: FeS-thio- and glutaredoxins☆ - p. Page 209 23. Chapter 12 : Glutathione and glutathione-dependent enzymes - p. Page 232 24. Chapter 13 : Thiol- and selenol-based peroxidases: Structure and catalytic properties - p. Page 261 25. Chapter 14 : Thiol peroxidase-based redox relays - p. Page 294 26. Chapter 15 : Compartmentalized disulfide bond formation pathways - p. Page 316 27. Chapter 16 : Disulfide bond formation in Escherichia coli - p. Page 336 28. Chapter 17 : Thiol-based redox probes - p. Page 361 29. Chapter 18 : Selenocysteine-containing proteins - p. Page 396 30. Chapter 19 : Overview of cysteine metabolism - p. Page 418 31. Chapter 20 : Hydrogen sulfide and persulfides - p. Page 441 32. Chapter 21 : The role of thiols in iron–sulfur cluster biogenesis - p. Page 477 33. Chapter 22 : Thiol-based redox control in chloroplasts - p. Page 499 34. Chapter 23 : Sugar-based cysteine thiols recruited for oxidative stress defense and redox regulation - p. Page 525 35. Chapter 24 : Polyamine-based thiols in pathogens - p. Page 549 36. Chapter 25 : Thiols in blood - p. Page 572 37. Chapter 26 : A thiol chemistry perspective on redox medicine - p. Page 604 38. Chapter 27 : Therapeutic applications of low-molecular-weight thiols and selenocompounds - p. Page 635 39. Chapter 28 : Thiol targets in drug development to combat bacterial infections - p. Page 664 40. Index - p. Page 699
