1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter One: Kinetic analysis of the three-substrate reaction mechanism of an NRPS-independent siderophore (NIS) synthetase - p. 2 8. Chapter Two: Experimental methods for evaluating siderophore–antibiotic conjugates - p. 22 9. Chapter Three: A continuous fluorescence assay to measure nicotianamine synthase activity - p. 52 10. Chapter Four: ITC-based kinetics assay for NIS synthetases - p. 76 11. Chapter Five: An in vitro assay to explore condensation domain specificity from non-ribosomal peptide synthesis - p. 91 12. Chapter Six: The production of siderophore analogues using precursor-directed biosynthesis - p. 122 13. Chapter Seven: Preparation of coenzyme F430 biosynthetic enzymes and intermediates - p. 148 14. Chapter Eight: Purification and biochemical characterization of methanobactin biosynthetic enzymes - p. 172 15. Chapter Nine: Discovery, isolation, and characterization of diazeniumdiolate siderophores - p. 190 16. Chapter Ten: Linking biosynthetic genes to natural products using inverse stable isotopic labeling (InverSIL) - p. 216 17. Chapter Eleven: 4-Aldrithiol-based photometric assay for detection of methylthioalkylmalate synthase activity - p. 230 18. Chapter Twelve: Methods for biochemical characterization of flavin-dependent N-monooxygenases involved in siderophore biosynthesis - p. 249 19. Chapter Thirteen: Siderophore-dependent ferrichelatases - p. 283 20. Chapter Fourteen: Native metabolomics for mass spectrometry-based siderophore discovery - p. 318 21. Chapter Fifteen: Anaerobic heme recycling by gut microbes: Important methods for monitoring porphyrin production - p. 354 22. Chapter Sixteen: Predicting metallophore structure and function through genome mining - p. 372
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter One: Kinetic analysis of the three-substrate reaction mechanism of an NRPS-independent siderophore (NIS) synthetase - p. 2 8. Chapter Two: Experimental methods for evaluating siderophore–antibiotic conjugates - p. 22 9. Chapter Three: A continuous fluorescence assay to measure nicotianamine synthase activity - p. 52 10. Chapter Four: ITC-based kinetics assay for NIS synthetases - p. 76 11. Chapter Five: An in vitro assay to explore condensation domain specificity from non-ribosomal peptide synthesis - p. 91 12. Chapter Six: The production of siderophore analogues using precursor-directed biosynthesis - p. 122 13. Chapter Seven: Preparation of coenzyme F430 biosynthetic enzymes and intermediates - p. 148 14. Chapter Eight: Purification and biochemical characterization of methanobactin biosynthetic enzymes - p. 172 15. Chapter Nine: Discovery, isolation, and characterization of diazeniumdiolate siderophores - p. 190 16. Chapter Ten: Linking biosynthetic genes to natural products using inverse stable isotopic labeling (InverSIL) - p. 216 17. Chapter Eleven: 4-Aldrithiol-based photometric assay for detection of methylthioalkylmalate synthase activity - p. 230 18. Chapter Twelve: Methods for biochemical characterization of flavin-dependent N-monooxygenases involved in siderophore biosynthesis - p. 249 19. Chapter Thirteen: Siderophore-dependent ferrichelatases - p. 283 20. Chapter Fourteen: Native metabolomics for mass spectrometry-based siderophore discovery - p. 318 21. Chapter Fifteen: Anaerobic heme recycling by gut microbes: Important methods for monitoring porphyrin production - p. 354 22. Chapter Sixteen: Predicting metallophore structure and function through genome mining - p. 372
