ZIP Metal Ion Transporters, Volume 684 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Biochemistry, Biophysics and Structural Biology, Structure determination of a bacterial ZIP in lipidic cubic phase, Cell-based metal transport assay for animal and plant ZIPs, Measurement of metal binding affinity and transport activity in ZIP transporters using spectroscopic methods, Considerations in production of the (prokaryotic) ZIP family transporters for structural and functional studies, Metal-protein interactions investigated using model systems: thermodynamic, spectroscopic and computational methods, and more.
Additional chapters cover Getting Zinc Into and Out of Cells, In-silico modeling of ZIP9 (TBD), Cellular Function and Regulation, Methods to visualize zinc transporter proteins of the SLC39A family in cells, Assessing metal ion transporting activity of ZIPs: intracellular zinc and iron detection, In vitro studies for studying manganese transport and homeostasis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter One: Considerations in production of the prokaryotic ZIP family transporters for structural and functional studies - p. 1 8. CHAPTER TWO: Expression, purification, crystallization of a ZIP metal transporter from Bordetella bronchiseptica (BbZIP) - p. 31 9. Chapter Three: Expression, purification, and crystallization of the extracellular domain of a mammalian ZIP4 - p. 49 10. Chapter Four: Methods to visualise zinc transporter proteins of the SLC39A family in cells - p. 67 11. Chapter Five: A simple and highly reproducible method for the detection of erythrocyte membrane ZIP metal transporters by immunoblotting - p. 87 12. Chapter Six: Single-molecule quantification of the oligomeric state of ZIP transporters in mammalian cells with fluorescence correlation spectroscopy - p. 104 13. Chapter Seven: Cell-based transport assay to study kinetics and substrate specificity of human ZIPs - p. 139 14. Chapter Eight: Assessing metal ion transporting activity of ZIPs: Intracellular zinc and iron detection - p. 157 15. Chapter Nine: In vitro studies of manganese transport and homeostasis - p. 186 16. Chapter Ten: Simple in vitro method to evaluate ZIP zinc transport ability through zinc transporter 1 and metallothionein expression measurements - p. 208 17. Chapter Eleven: Internalization and trafficking of zinc transporters - p. 241 18. Chapter Twelve: Getting zinc into and out of cells - p. 263 19. Chapter Thirteen: Metal–protein solution interactions investigated using model systems: Thermodynamic and spectroscopic methods - p. 280
ZIP Metal Ion Transporters, Volume 684 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Biochemistry, Biophysics and Structural Biology, Structure determination of a bacterial ZIP in lipidic cubic phase, Cell-based metal transport assay for animal and plant ZIPs, Measurement of metal binding affinity and transport activity in ZIP transporters using spectroscopic methods, Considerations in production of the (prokaryotic) ZIP family transporters for structural and functional studies, Metal-protein interactions investigated using model systems: thermodynamic, spectroscopic and computational methods, and more.
Additional chapters cover Getting Zinc Into and Out of Cells, In-silico modeling of ZIP9 (TBD), Cellular Function and Regulation, Methods to visualize zinc transporter proteins of the SLC39A family in cells, Assessing metal ion transporting activity of ZIPs: intracellular zinc and iron detection, In vitro studies for studying manganese transport and homeostasis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter One: Considerations in production of the prokaryotic ZIP family transporters for structural and functional studies - p. 1 8. CHAPTER TWO: Expression, purification, crystallization of a ZIP metal transporter from Bordetella bronchiseptica (BbZIP) - p. 31 9. Chapter Three: Expression, purification, and crystallization of the extracellular domain of a mammalian ZIP4 - p. 49 10. Chapter Four: Methods to visualise zinc transporter proteins of the SLC39A family in cells - p. 67 11. Chapter Five: A simple and highly reproducible method for the detection of erythrocyte membrane ZIP metal transporters by immunoblotting - p. 87 12. Chapter Six: Single-molecule quantification of the oligomeric state of ZIP transporters in mammalian cells with fluorescence correlation spectroscopy - p. 104 13. Chapter Seven: Cell-based transport assay to study kinetics and substrate specificity of human ZIPs - p. 139 14. Chapter Eight: Assessing metal ion transporting activity of ZIPs: Intracellular zinc and iron detection - p. 157 15. Chapter Nine: In vitro studies of manganese transport and homeostasis - p. 186 16. Chapter Ten: Simple in vitro method to evaluate ZIP zinc transport ability through zinc transporter 1 and metallothionein expression measurements - p. 208 17. Chapter Eleven: Internalization and trafficking of zinc transporters - p. 241 18. Chapter Twelve: Getting zinc into and out of cells - p. 263 19. Chapter Thirteen: Metal–protein solution interactions investigated using model systems: Thermodynamic and spectroscopic methods - p. 280
