Antimicrobial Peptides, Volume 663 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Unifying the classification of antimicrobial peptides in the Antimicrobial Peptide Database, Optimizing peptide library creation for PepSAVI-MS (RP libraries, etc.), Discovery of novel Antimicrobial peptides using BioProspecting, Screening for cysteine-stabilized scaffolds for developing protelytic-resistant AMPs, Exploring synergy and its role in antimicrobial peptide biology, Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens, and much more.
Other chapters cover Liquid chromatography-mass spectrometry-based analysis of naturally occurring neuropeptide diastereomers, Multiplexed Quantitative Neuropeptidomics via DiLeu Isobaric Tagging, In vitro evaluation of antibiotic resistance via proteomics, Molecular networking-based strategies in mass spectrometry, Development of Macrocyclic antimicrobial peptides and peptoids, and a host of other timely topics.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Chapter One: Unifying the classification of antimicrobial peptides in the antimicrobial peptide database - p. 14 7. Chapter Two: Protocols for measuring the stability and cytotoxicity of cyclotides - p. 19 8. Chapter Three: Creating optimized peptide libraries for AMP discovery via PepSAVI-MS - p. 41 9. Chapter Four: Screening for cysteine-stabilized scaffolds for developing proteolytic-resistant AMPs - p. 67 10. Chapter Five: Exploring synergy and its role in antimicrobial peptide biology - p. 99 11. Chapter Six: Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens - p. 137 12. Chapter Seven: In silico prediction and mass spectrometric characterization of botanical antimicrobial peptides - p. 157 13. Chapter Eight: Combined thermal and carboxypeptidase Y stability assays for probing the threaded fold of lasso peptides - p. 177 14. Chapter Nine: Evaluation of endogenous peptide stereochemistry using liquid chromatography-mass spectrometry-based spiking experiments - p. 205 15. Chapter Ten: Multiplexed quantitative neuropeptidomics via DiLeu isobaric tagging - p. 235 16. Chapter Eleven: Revealing AMP mechanisms of action through resistance evolution and quantitative proteomics - p. 259 17. Chapter Twelve: Molecular networking-based strategies in mass spectrometry coupled with in silico dereplication of peptidic natural products and gene cluster analysis - p. 284 18. Chapter Thirteen: Methods for the design and characterization of peptide antibiotics - p. 303 19. Chapter Fourteen: Solid-phase synthesis of novel antimicrobial peptoids with α- and β-chiral side chains - p. 327 20. Chapter Fifteen: Molecular networking in infectious disease models - p. 341
Antimicrobial Peptides, Volume 663 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Unifying the classification of antimicrobial peptides in the Antimicrobial Peptide Database, Optimizing peptide library creation for PepSAVI-MS (RP libraries, etc.), Discovery of novel Antimicrobial peptides using BioProspecting, Screening for cysteine-stabilized scaffolds for developing protelytic-resistant AMPs, Exploring synergy and its role in antimicrobial peptide biology, Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens, and much more.
Other chapters cover Liquid chromatography-mass spectrometry-based analysis of naturally occurring neuropeptide diastereomers, Multiplexed Quantitative Neuropeptidomics via DiLeu Isobaric Tagging, In vitro evaluation of antibiotic resistance via proteomics, Molecular networking-based strategies in mass spectrometry, Development of Macrocyclic antimicrobial peptides and peptoids, and a host of other timely topics.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Chapter One: Unifying the classification of antimicrobial peptides in the antimicrobial peptide database - p. 14 7. Chapter Two: Protocols for measuring the stability and cytotoxicity of cyclotides - p. 19 8. Chapter Three: Creating optimized peptide libraries for AMP discovery via PepSAVI-MS - p. 41 9. Chapter Four: Screening for cysteine-stabilized scaffolds for developing proteolytic-resistant AMPs - p. 67 10. Chapter Five: Exploring synergy and its role in antimicrobial peptide biology - p. 99 11. Chapter Six: Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens - p. 137 12. Chapter Seven: In silico prediction and mass spectrometric characterization of botanical antimicrobial peptides - p. 157 13. Chapter Eight: Combined thermal and carboxypeptidase Y stability assays for probing the threaded fold of lasso peptides - p. 177 14. Chapter Nine: Evaluation of endogenous peptide stereochemistry using liquid chromatography-mass spectrometry-based spiking experiments - p. 205 15. Chapter Ten: Multiplexed quantitative neuropeptidomics via DiLeu isobaric tagging - p. 235 16. Chapter Eleven: Revealing AMP mechanisms of action through resistance evolution and quantitative proteomics - p. 259 17. Chapter Twelve: Molecular networking-based strategies in mass spectrometry coupled with in silico dereplication of peptidic natural products and gene cluster analysis - p. 284 18. Chapter Thirteen: Methods for the design and characterization of peptide antibiotics - p. 303 19. Chapter Fourteen: Solid-phase synthesis of novel antimicrobial peptoids with α- and β-chiral side chains - p. 327 20. Chapter Fifteen: Molecular networking in infectious disease models - p. 341
