Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. xv 7. Section 1: Methods for studying the catalytic mechanisms of mononuclear non-heme iron dependent enzymes - p. 1 8. Chapter One: Characterization of O2 uncoupling in biodegradation reactions of nitroaromatic contaminants catalyzed by rieske oxygenases - p. 3 9. Chapter Two: Spectroscopic definition of ferrous active sites in non-heme iron enzymes - p. 29 10. Chapter Three: Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase - p. 51 11. Chapter Four: Preparation of reductases for multicomponent oxygenases - p. 65 12. Chapter Five: Development of a rapid mass spectrometric method for the analysis of ten-eleven translocation enzymes - p. 87 13. Chapter Six: Non-standard amino acid incorporation into thiol dioxygenases - p. 121 14. Chapter Seven: Unveiling the mechanism of cysteamine dioxygenase: A combined HPLC-MS assay and metal-substitution approach - p. 147 15. Chapter Eight: In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01 - p. 168 16. Section 2: Leveraging mononuclear non-heme iron enzymes for biocatalysis - p. 193 17. Chapter Nine: Radical-relay C(sp3)–H azidation catalyzed by an engineered nonheme iron enzyme - p. 195 18. Chapter Ten: Purification and characterization of a Rieske oxygenase and its NADH-regenerating partner proteins - p. 215 19. Chapter Eleven: Whole-cell Rieske non-heme iron biocatalysts - p. 243 20. Chapter Twelve: Photo-reduction facilitated stachydrine oxidative N-demethylation reaction: A case study of Rieske non-heme iron oxygenase Stc2 from Sinorhizobium meliloti - p. 263 21. Chapter Thirteen: Functional and spectroscopic approaches to determining thermal limitations of Rieske oxygenases - p. 299
Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. xv 7. Section 1: Methods for studying the catalytic mechanisms of mononuclear non-heme iron dependent enzymes - p. 1 8. Chapter One: Characterization of O2 uncoupling in biodegradation reactions of nitroaromatic contaminants catalyzed by rieske oxygenases - p. 3 9. Chapter Two: Spectroscopic definition of ferrous active sites in non-heme iron enzymes - p. 29 10. Chapter Three: Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase - p. 51 11. Chapter Four: Preparation of reductases for multicomponent oxygenases - p. 65 12. Chapter Five: Development of a rapid mass spectrometric method for the analysis of ten-eleven translocation enzymes - p. 87 13. Chapter Six: Non-standard amino acid incorporation into thiol dioxygenases - p. 121 14. Chapter Seven: Unveiling the mechanism of cysteamine dioxygenase: A combined HPLC-MS assay and metal-substitution approach - p. 147 15. Chapter Eight: In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01 - p. 168 16. Section 2: Leveraging mononuclear non-heme iron enzymes for biocatalysis - p. 193 17. Chapter Nine: Radical-relay C(sp3)–H azidation catalyzed by an engineered nonheme iron enzyme - p. 195 18. Chapter Ten: Purification and characterization of a Rieske oxygenase and its NADH-regenerating partner proteins - p. 215 19. Chapter Eleven: Whole-cell Rieske non-heme iron biocatalysts - p. 243 20. Chapter Twelve: Photo-reduction facilitated stachydrine oxidative N-demethylation reaction: A case study of Rieske non-heme iron oxygenase Stc2 from Sinorhizobium meliloti - p. 263 21. Chapter Thirteen: Functional and spectroscopic approaches to determining thermal limitations of Rieske oxygenases - p. 299
