The Design and Development of Novel Drugs and Vaccines: Principles and Protocols presents both in silico methods and experimental protocols for vaccine and drug design and development, critically reviewing the most current research and emphasizing approaches and technologies that accelerate and lower the cost of product development. Sections review the technologies and approaches used to identify, characterize and establish a protein as a new drug and vaccine target, cover several molecular methods for in vitro studies of the desired target, and present various physiological parameters for in vivo studies. The book includes preclinical trials and research, along with information on FDA approval.
1. Chapter 1 : Introduction of structural bioinformatics with respect to drug discovery - p. Page ix 2. Chapter 2 : Target identification and validation - p. Page 7 3. Chapter 3 : Retrieval of compounds - p. Page 15 4. Chapter 4 : Lead identification and optimization - p. Page 27 5. Chapter 5 : Molecular dynamics simulations - p. Page 61 6. Chapter 6 : An overview of vaccine design - p. Page 80 7. Chapter 7 : Key steps in the selection of vaccine targets - p. Page 90 8. Chapter 8 : Epitope prediction and selection of linkers and adjuvant - p. Page 95 9. Chapter 9 : Design of vaccine constructs - p. Page 106 10. Chapter 10 : In silico validation through protein-protein docking - p. Page 118 11. Chapter 11 : Molecular cloning - p. Page 131 12. Chapter 12 : Protein expression - p. Page 161 13. Chapter 13 : Protein purification and desalting - p. Page 180 14. Chapter 14 : Biological assay - p. Page 200 15. Chapter 15 : Biophysical methods - p. Page 210 16. Chapter 16 : Outline of animal study for lead/vaccine testing - p. Page 220 17. Chapter 17 : Evaluation of immunogenicity of vaccine candidates - p. Page 231 18. Chapter 18 : In-vitro evaluation of lead molecule - p. Page 237 19. Chapter 19 : Medical physiological parameter for lead/vaccine testing - p. Page 245 20. Chapter 20 : Brief introduction of clinical research and trials - p. Page 258 21. Chapter 21 : An overview of IND, NDA, approval agencies and FDA post-marketing surveillance - p. Page 265 22. Chapter 22 : Introduction of intellectual property rights - p. Page 272 23. Index - p. Page 281
The Design and Development of Novel Drugs and Vaccines: Principles and Protocols presents both in silico methods and experimental protocols for vaccine and drug design and development, critically reviewing the most current research and emphasizing approaches and technologies that accelerate and lower the cost of product development. Sections review the technologies and approaches used to identify, characterize and establish a protein as a new drug and vaccine target, cover several molecular methods for in vitro studies of the desired target, and present various physiological parameters for in vivo studies. The book includes preclinical trials and research, along with information on FDA approval.
1. Chapter 1 : Introduction of structural bioinformatics with respect to drug discovery - p. Page ix 2. Chapter 2 : Target identification and validation - p. Page 7 3. Chapter 3 : Retrieval of compounds - p. Page 15 4. Chapter 4 : Lead identification and optimization - p. Page 27 5. Chapter 5 : Molecular dynamics simulations - p. Page 61 6. Chapter 6 : An overview of vaccine design - p. Page 80 7. Chapter 7 : Key steps in the selection of vaccine targets - p. Page 90 8. Chapter 8 : Epitope prediction and selection of linkers and adjuvant - p. Page 95 9. Chapter 9 : Design of vaccine constructs - p. Page 106 10. Chapter 10 : In silico validation through protein-protein docking - p. Page 118 11. Chapter 11 : Molecular cloning - p. Page 131 12. Chapter 12 : Protein expression - p. Page 161 13. Chapter 13 : Protein purification and desalting - p. Page 180 14. Chapter 14 : Biological assay - p. Page 200 15. Chapter 15 : Biophysical methods - p. Page 210 16. Chapter 16 : Outline of animal study for lead/vaccine testing - p. Page 220 17. Chapter 17 : Evaluation of immunogenicity of vaccine candidates - p. Page 231 18. Chapter 18 : In-vitro evaluation of lead molecule - p. Page 237 19. Chapter 19 : Medical physiological parameter for lead/vaccine testing - p. Page 245 20. Chapter 20 : Brief introduction of clinical research and trials - p. Page 258 21. Chapter 21 : An overview of IND, NDA, approval agencies and FDA post-marketing surveillance - p. Page 265 22. Chapter 22 : Introduction of intellectual property rights - p. Page 272 23. Index - p. Page 281
